|Abstract: ||豆粕由於蛋白質含量高、價廉且供應穩定, 為重要蛋白質供應者，已知去醣基大豆異黃酮及胜肽具抗肥胖活性。本研究分別以 Lactobacillus sp. FPS 2520 及 Bacillus sp. N1菌株進行單株及混合菌株液態發酵豆粕，探討發酵豆粕產品之抗肥胖活性。以五週齡 Sprague-Dawley 大鼠，隨機分為 9 組：(1) Normal diet 組 (ND)，其餘八組以高脂飼料誘導肥胖分為 (2) High-Fat diet 控制組 (HFD)，(3) HFD + 未發酵豆粕 1000 mg/kg 組 (SM 1000)，(4) 和 (5) HFD + FPS 2520 發酵產品，劑量分別為 500、1000 mg/kg 組 (FPS 500、FPS 1000)，(6) 和 (7) HFD + N1 發酵產品，劑量分別為 500、1000 mg/kg 組 (N1 500、N1 1000)，(8) 和 (9) HFD + 複合菌株發酵產品，劑量分別為 500、1000 mg/kg 組 (Mix 500、Mix 1000)，SD 大鼠經十週高脂飼料誘導肥胖後，餵食各測試樣品六週，顯示各實驗組除了 SM 1000 組外，體重均顯著低於HFD組，尤以 Mix 1000 組下降最大，與 ND 組相近。大鼠腹部及副睪脂肪含量變化亦和體重變化相似且具劑量依賴性，Mix 1000 組之腹部及副睪脂肪量分別比 HFD 組下降 47.7% 及 41.1%。各測試發酵產物均顯著降低血漿中三酸甘油脂及總膽固醇含量，Mix 1000 組比HFD 組下降 11.1% 及 29.8%。各測試發酵產物均降低血漿中 Alanine aminotransferase (ALT)、Aspartate aminotransferase (AST) 及 Creatinine 濃度。此外，各式菌株發酵產物均可降低脂肪酸合成酶 (FAS)、乙醯輔酶 A 羧化酶 (ACC) 酵素活性及增加荷爾蒙敏感性脂解酶 (HSL)、脂蛋白脂肪酶 (LPL) 酵素活性，且均以 Mix 1000 組的效果最大。綜合上述，由 FPS 2520 及 N1單株或複合菌株發酵所得豆粕均具降低脂質累積能力之抗肥胖活性，其中尤以複合菌株發酵的效果最顯著。|
Because of its high protein content, low cost and stable supply, soybean meal (SBM) is an important protein source. It is known that deglycosyl soy isoflavones and peptides have anti-obesity activity. In this study, the single strain or mixed strains of Lactobacillus sp. FPS 2520 and Bacillus sp. N1 were used to ferment SBM, and the anti-obesity activity of the fermented SBM products were investigated using a high fat-induced animal model. Five-week-old Sprague-Dawley rats were randomly divided into 9 groups: (1) Normal diet group (ND), and the remaining 8 groups were divided into high-fat diet-induced obesity including (2) High-Fat diet control group (HFD), ( 3) HFD + unfermented soybean meal 1000 mg/kg group (SM 1000), (4) & (5) HFD + FPS 2520 fermented products at dosage of 500 and 1000 mg/kg, respectively, (FPS 500, FPS 1000), (6) & (7) HFD + N1 fermented products at dosage of 500 and 1000 mg/kg, respectively, (N1 500, N1 1000), (8) & (9) HFD + mixed strain fermented products at dosage of 500 and 1000 mg/kg, respectively, (Mix 500, Mix 1000), SD rats were successfully induced to become obesity after 10 weeks of induction by high fat diet, and then, the various tested fermented products were orally ingested by 6 weeks. Except the SM 1000 group, the body weight gains for the rest experimental groups were significantly lower than that of HFD group, among which Mix 1000 group being the lowest, with 25.8% reduction, compared to that of HFD. The changes for abdominal fat weight and epididymis fat weight were also similar to that of body weight gain, with Mix 1000 group being lowest, with 47.7% and 41.1% reduction, respectively, compared to those of HFD. All the tested fermented products significantly reduced the levels of plasma triglyceride and total cholesterol with 11.1% and 29.8% reduction were obtained for Mix 1000 group, compared to HFD group. Similarly, the plasma alanine aminotransferase (ALT), Aspartate aminotransferase (AST) and creatinine levels were decreased for all experimented groups. The activity of fatty acid synthase (FAS) and acetyl CoA carboxylase (ACC) in liver were significantly decreased, and the activity of hormone sensitive lipase (HSL) and lipoprotein lipase (LPL) in adipose tissue were significantly decreased, for the groups fed with fermented SBM, with Mix 1000 group being most. In summary, the fermented SBM by either FPS 2520 or N1 were demonstrated to have anti-obesity activity, with mix strain fermented SBM being the most.