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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/53584

Title: 臺灣東北角瑪鋉溪之洄游型臺灣特有種臺灣吻鰕虎的生殖生物學與全粒線體基因研究
Reproductive biology and complete mitochondrial genome of endemic amphidromous goby Rhinogobius formosanus (Teleostei: Gobiidae) from the Masu River, Northern Taiwan
Authors: Tonisman Harefa
方威廷
Contributors: NTOU:Institute of Marine Biology
國立臺灣海洋大學:海洋生物研究所
Keywords: 生殖生物學;繁殖力;Rhinogobius;完整的基因組;系統發育
Reproductive biology;fecundity;Rhinogobius;complete genome;phylogeny
Date: 2019
Issue Date: 2020-07-03T08:34:12Z
Abstract: 台灣吻鰕虎Rhinogobius formosanus Oshima,1919為台灣特有種淡水鰕虎,屬於降海洄游物種,在台灣北部分佈較窄,被列為近瀕危物種。本研究目的為調查台灣北部新北市瑪鋉溪2018年3月至2019年3月,台灣吻鰕虎每月之生殖研究。本次實驗共調查到355隻台灣吻鰕虎,包括180隻雌魚和155隻雄魚。 雌魚的總長範圍為21.17至70.76毫米,雄魚的總長範圍為28.54至72.12毫米。雌性和雄性的性別比為1.16:1。根據GSI的每月變化,估計產卵季節發生在2月至4月。成熟雌魚的絕對孕卵數為806至2955,平均為1,489±468,隨著魚的長度和體重顯著增加。卵母細胞平均大小為0.51mm。初次成熟時的雌魚長度為44.18mm,雄魚為47.48mm。長度 - 重量關係描述了這種鰕虎的生長模式是異速生長的。魚類粒線體基因對於了解它們的分子演化和系統發育關係非常重要。台灣吻鰕虎的完整粒線體基因組序列長度為16,502 bp,含有37個基因,其中22個tRNA,2個rRNA,13個PCG,一個複制起點(OL)和一個控制區域。DNA鹼基組成為A 26.2%、C 30.5%、T 25.6%、G 17.7%。依據完整的粒線體序列,利用最大似然和貝葉斯推斷方法構建演化樹。兩種樹狀拓撲都高度支持台灣吻鰕虎Rhinogobius formosanus和大吻鰕虎Rhinogobius brunneus之間的關係。
The freshwater goby Rhinogobius formosanus Oshima, 1919 is one of endemic fish, listed as being near threatened species and has a narrow distribution in the northern Taiwan although it belongs to amphidromous species. Present study aimed to investigate reproductive aspects through monthly collection from March 2018 to March 2019 from the Masu River, New Taipei City, North Taiwan. Overall 355 fish gobies were examined including 180 females and 155 males. Total length range for females was 21.17 to 70.76 mm and for males was 28.54 to 72.12 mm. The overall sex ratio between female and male was 1.16: 1. Spawning season was estimated to occur in February-April, based on the monthly change of GSI. Absolute fecundity of mature female ranged from 806 to 2955 with an average 1,489 ± 468, showing a significant increase with length and body weight of fish. Oocyte size averaged was 0.51mm. Female length at first maturity was 44.18mm and for male was 47.48mm. Length-weight relationship described growth pattern of this fish goby was allometric negative. Fish mitochondrial genome is very important to understand their molecular evolution and phylogenetic relationships. The complete mitochondrial genome sequence of R. formosanus was 16,502 bp in length, and contained 37 genes with 22 tRNAs, 2 rRNAs, 13 PCGs, one origin of replication (OL) and one control region in this mitogenomes. The overall of base composition was 26,2 % for A, 30,5% for C, 25,6% for T and 17,7% for G. Phylogenetic tree was constructed with Maximum likelihood and Bayesian Inference method based on complete mitogenome sequences. Both tree topologies highly supported the relationship between R. formosanus and R. brunneus.
URI: http://ethesys.lib.ntou.edu.tw/cgi-bin/gs32/gsweb.cgi?o=dstdcdr&s=G0010634017.id
http://ntour.ntou.edu.tw:8080/ir/handle/987654321/53584
Appears in Collections:[海洋生物研究所] 博碩士論文

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