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Title: 不同培養因子對頂絲藻(Acrochaetium sp.)成長及藻膽蛋白之影響
The effect of different culture factors on the growth and phycobiliprotein content of Acrochaetium sp.
Authors: Jhang, Fu-Jie
Contributors: NTOU:Department of Aquaculture
Keywords: 頂絲藻;藻膽蛋白;藻紅蛋白;培養因子;特殊成長率
Acrochaetium;Phycobiliprotein;Phycoerythrin;Culture factor;Specific growth rate
Date: 2019
Issue Date: 2020-07-03T08:30:24Z
Abstract: 本研究探討之頂絲藻(Acrochaetium sp.)為臺灣原生種大型紅藻,藻種於先前的成份分析研究發現其具有豐富的蛋白質含量,極具產業應用價值。為有效的生產藻體用以提取高品質的藻紅蛋白,本研究嘗試分析培養因子溫度、照度、光照週期以及光照質量對頂絲藻藻體生長及其藻膽蛋白之組成變化與含量的影響,建立頂絲藻大量人工養殖與最佳藻紅蛋白量產技術,提供高品質穩定的藻紅蛋白,振興我國海藻養殖產業。 培養因子溫度與照度試驗結果顯示,頂絲藻培養於 26℃、60 及100 μmol photons m-2s-1 時有最佳的特殊成長率分別為 14.62  0.31、14.50  0.11% day-1,其次為 22℃、100 μmol photons m-2s-1,特殊成長率達 13.23  0.47% day-1,但成長較26℃穩定並有最高純度的藻紅蛋白佔比,達 65.44 ± 1.13%。另一方面,光照週期的日照長度與藻體生長率呈正相關,並以 16L:8D 為最佳,其藻體特殊成長率達 13.29  0.05% day-1。值得注意的是,僅藻藍蛋白之含量隨著光照週期日照長度增加而遞增,光照週期對其餘各式藻膽蛋白及總藻膽蛋白之生成含量無顯著影響。 在光照質量試驗中,以白光光源培養頂絲藻有最佳的藻體生長表現,其特殊成長率達 13.76  0.29% day-1。有趣的是,藻體中各式藻膽蛋白之含量及佔總藻膽蛋白之比例皆受不同光照質量影響。以紅光培養頂絲藻有最佳的藻藍蛋白生成量達 1.94  0.11 mg g-1 並佔總藻膽蛋白比例達 30.20  0.85%。此外,藍光及綠光培養頂絲藻有最佳的藻紅蛋白生成量分別達 5.12  0.15 與 4.78  0.11 mg g-1。最後,藍光培養頂絲藻有較高的異藻藍蛋白生成量達 1.86  0.19 mg g-1 並佔總藻膽蛋白比例達 20.81  0.03%,於此條件下亦有最高的總藻膽蛋白生成,生成含量達 8.94  0.35 mg g-1。
Acrochaetium sp., a recognized red seaweed species native from Taiwan. It has been considered as a rich protein source with promising industrial potential. To continuously promote its utilization in commercial scale, the development of cultivation technology outlining efficient biomass with higher productivity is a challenge. In this study, effects of temperature (14, 18, 22 and 26℃), light intensity (20, 60 and 100 μmol photons m-2s-1), photoperiod (8L:16D, 12L:12D and 16L:8D) and light quality (white, red, green and blue) in the experimental conditions of Acrochaetium sp. was investigated. Under these environmental conditions that designed separately in the experiment, various parameters including growth, and photosynthetic pigments [phycobiliprotein (PBP), phycoerythrin (PE), phycocyanin (PC), total phycobiliprotein (TPBP), and allophycocyanin (APC)] were measured. The results showed that specific growth rate (SGR) was enhanced at 26℃, 60 and 100 μmol photons m-2 s-1 with values of 14.62  0.31, 14.50  0.11% day-1 were achieved, respectively. Thereafter 22℃, 100 μmol photons m-2 s-1 obtained highly consistent value with 13.23 ± 0.47% day-1 compared with 26℃. In addition, PE was recored the highest purity value accounted to 65.44 ± 1.13%. On the other hand, the photoperiod was positively correlated with the growth rate of the algae and is best reflected at 16L:8D conditions with 13.29 ± 0.05% day-1 SGR. Among the photosynthetic pigments, it appeared that only PC was affected by photoperiod and were significantly increased with increasing photoperiod. With regards to light quality, results revealed that the best growth performance with an SGR value of 13.76 ± 0.29% day-1 was obtained in white light. Interestingly, the contents of PBP and the proportion of TPBP in the algae were affected by different light quality. While culture algae in the red light obtained the optimum PC with 1.94 ± 0.11 mg g-1, algae cultured in blue light and green light obtained the optimum PE at 5.12 ± 0.15 and 4.78 ± 0.11 mg g-1, respectively. It is also worth noting that high content of APC was found in the blue light with a calculated 1.86 ± 0.19 mg g-1 corresponding to 20.81 ± 0.03% of TPBP, and similarly obtained the highest TPBP content at 8.94 ± 0.35 mg g-1 under these conditions.
URI: http://ethesys.lib.ntou.edu.tw/cgi-bin/gs32/gsweb.cgi?o=dstdcdr&s=G0010733002.id
Appears in Collections:[水產養殖學系] 博碩士論文

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