|Abstract: ||本試驗旨在釐清齒額米蝦(Caridina serratirostris）蝦苗培育之最適生長條件，並且建立齒額米蝦幼蝦發育期數變化判定標準，提供將來商業化養殖或是台灣原生種保育的基礎。所有試驗均在2L燒杯中進行。蝦苗起始密度為30隻/1.6升，光照週期為12L:12D，水溫控制在25±1℃。分別就鹽度、微藻種類以及微藻飼育密度對蝦苗幼生活存與成長之影響。並比較人工完全養殖子代與野生子代幼苗活存與成長之差異。鹽度試驗：將眼幼蟲置入鹽度各為15, 20, 25, 30‰ (三重複)並飼以等密度 (1.5×105cell/ml) 之周氏扁藻(Tetraselmis chui)。微藻種類飼育試驗：分別將眼幼蟲置於等密度 (1.5×105cell/ml)之牟氏角毛藻(Chaetocero muelleri)、等鞭金藻(Isochrysis galbana)、周氏扁藻(T. chui)、魏氏海鏈藻(Thalassiosira weissflogii) (三重複)進行育苗試驗。藻類密度試驗：牟氏角毛藻(C. muelleri) 密度分別為(0.5、1、2、4、8) ×105cell/ml與周氏扁藻(T. chui) 密度分別為 (0.5、1、2、4) ×105cell/ml進行育苗試驗。 稚蝦完全變態活存率結果顯示在鹽度25‰最高(50.0%)，顯著高於30‰ (28.9%)與20‰ (16.6%)及15‰ (2.2%)。餌料生物試驗周氏扁藻(T. chui) (31.1%)顯著高於牟氏角毛藻(C. muelleri) (5.3%)，而等鞭金藻(I. galbana)與魏氏海鏈藻(T. weissflogii)均無法使齒額米蝦幼蝦成功發育至後期幼蟲期。周氏扁藻(T. chui)在密度5×104cells/ml有最高的活存率(73.3%)顯著高於1×105cells/ml(50.0%), 2×105cells/ml(25.5%)以及4×105cells/ml(27.7%)者。牟氏角毛藻(C. muelleri)在密度2×105cells/ml有最高的活存率(33.3%)顯著高於1×105cells/ml(7.8%)並且5×104cells/ml(0%)與4×105cells/ml(0%)及8×105cells/ml(0%)皆無法使齒額米蝦幼蝦成功發育至後期幼蟲期。 本試驗結果顯示齒額米蝦蝦苗幼生培育最佳條件為：鹽度25‰，飼以密度5×104cells/ml之周氏扁藻(T. chui)為最佳。蝦苗發育期數分為九期(Zoea I-V , Mysis stage I- III , Post larvae)，幼苗變態至後期幼蟲天數為22 - 24天。|
The objective of this study is to investigate the optimal growth conditions for the larval culture of Ninja shrimp, Caridina serratirostris, so as to provide criteria guide line in seed production both for future commercial farming and wild population conservation. A series of experiments have been executed on the effects of salinity, microalgae species and microalgae density to do with the survival rate during larval culture. Additional larval survival and growth rate between the progenies of wild and artificially produced ones were compared. All the experiments initiated Zoea I stage at a density of 30 ind./L in a 2-liter beaker (3 replicates) with unified photoperiod (12L:12D), temperature (25±1℃) conditional parameters. Result of feeding larvae with Tetraselmis chui (1.5x105 cells/ml) showed that the highest larval metamorphic survival rate (50%) occurred at a salinity of 25‰ significantly higher than that at 30‰ (28.9%), 20‰ (16.6%) and 15 ‰ (2.2%, the lowest). Of the four Microalgal species, feeing ninja shrimp larvae at density of 1.5×105cell/ml found that T. chui exhibited highest metamorphic survival rate (31.1%) significantly higher than that group fed Chaetocero muelleri (5.3%). All shrimp larvae fed Isochrysis galbana and Thalassiosira weissflogii died at various stages before metamorphosis. Of the algal density trial, five density levels of C. muelleri (0.5, 1, 2, 4, 8) × 105 cell/ml and four for Tetraselmis chui (0.5, 1, 2, 4) × 105 cell/ml (triplicate) were fed to the larvae of each treatment. It was found that the highest survival rate (73.3 %) occurred at those larvae received T. chui density at 5×104 cells/ml which was significantly higher than the group at 1×105 cells/ml (50.0%), 4×105 cells/ml ( 27.7%) and 2×105 cells/ml (25.5%, the lowest); whilst, those received C. muelleri showed the highest survival rate (33.3%) at a density of 2×105 cells/ml significantly higher than the group at 1×105 cells/ml (7.8%). Zero survival rate occurred at too low (5×104 cells/ml) or too high (4×105, 8×105 cells/ml) of C. muelleri densities fed groups of larvae tested. It is concluded that the optimal condition set for culturing ninja shrimp larvae, C. serratirostris be: larval culture density (30 ind./L), T. chui feeding density (5×104 cells/ml), photoperiod (12L: 12D), temperature (25±1℃). Detailed developmental stages and their associated keys are described throughout the duration from Zoea I to post larva stage within 22 - 24 days of culture.