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|Title: ||不同溫度下烷烴降解菌屬(Alcanivorax sp.)之生長及柴油降解|
Growth and diesel degradation of Alcanivorax sp. under various temperatures
|Authors: ||Hu, Chen-Yi|
|Contributors: ||NTOU:Department of Environmental Biology and Fisheries Science|
Diesel;hydrocarbon-degrading bacterias;Alcanivorax sp.;temperature
|Issue Date: ||2020-07-02T07:40:34Z
|Abstract: ||本研究於八斗子漁港採集底泥及水樣，於含柴油之培養基內進行菌種單離，篩選出的菌種經過特性分析及 16S rDNA 進行定序鑑定，再依照不同柴油濃度 (1、2、5及10%) 及溫度 (20、25及30℃) 之條件進行培養，觀察菌數變化及柴油降解情況。採樣菌種經特性分析及定序得知為Alcanivorax sp. (烷烴降解菌屬)。相較於對照組，含有柴油之培養基中菌數皆有顯著增加 (p<0.05)。於5%柴油濃度培養6天後菌種生長情況顯著高於1%、2%及10%，而10%濃度下菌數成長較其他實驗組緩慢。實驗期間內5%柴油濃度之降解率最高，2% 及1%濃度次之，10%柴油濃度下之降解較不明顯。根據統計分析結果，不同溫度條件下之菌數在第3天後開始具有顯著差異 (p<0.05)，細菌於30℃環境下生長速率較高，20℃下則未觀察到細菌數有顯著成長。實驗7天後30℃之降解率最高，在第4天後不同溫度間之柴油濃度開始具有顯著差異 (p<0.05)，而結果顯示菌種之柴油降解率隨著環境溫度提高而增加。|
This study cellected sediments and water samples from Badouzi Fishing Port for isolating strains in diesel agar. The selected strain was characterized by characterization and 16S rDNA, then cultivating in different diesel concentrations (1, 2, 5 and 10%) and temperature conditions (20, 25 and 30°C). Observing changes in bacterial biomass and degradation of diesel.According to the characteristic and sequencing, the strain was confirmed to be Alcanivorax sp.. The bacterial biomass in diesel were significant higher than control group (p<0.05). The growth rate of bacteria cultured in 5% diesel was significant higher than the other treatments after 6 days, and the growth rate of bacteria at 10% diesel concentration was slower the other treatments. The degradation rate of 5% diesel concentration was the highest during the experiment period, followed by 2% and 1% concentration, and the degradation under 10% diesel concentration was not obviously.The amount of bacteria under different temperature conditions began to differ significantly after 3 days (p<0.05). The bacterial growth rate was the highest at 30°C, and there are no significant growth difference at 20°C. After 7 days of experiment, the highest degradation rate was found at 30°C. The diesel concentration at different temperatures had significanty difference after 4 days (p<0.05). This results showed the degradation rate of diesel was significant increased with ambient temperature (p<0.05).
|Appears in Collections:||[環境生物與漁業科學學系] 博碩士論文|
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