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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/52896

Title: Differential proteomics to explore the inhibitory effects of acidic, slightly acidic electrolysed water and sodium hypochlorite solution on Vibrio parahaemolyticus
Authors: Deng-Fwu Hwang
Shui-Tein Chen
Shu-Hao Kuo
Tai-Yuan Chen
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: Slightly acidic electrolysed water
Acidic electrolysed water
V. parahaemolyticusProteomic
Bactericidal mechanism
Date: 2016-05
Issue Date: 2020-01-20T08:52:00Z
Publisher: Scisence Journals
Abstract: Abstract: Slightly acidic electrolysed water (SlAEW) and acidic electrolysed water (AEW) have been demonstrated to effectively inactivate food-borne pathogens. However, the underlying mechanism of inactivation remains unknown. Therefore, in this study, a differential proteomic platform was used to investigate the bactericidal mechanism of SlAEW, AEW, and sodium hypochlorite (NaOCl) solutions against Vibrio parahaemolyticus. The upregulated proteins after SlAEW, AEW, and NaOCl treatments were identified as outer membrane proteins K and U. The downregulated proteins after the SlAEW, AEW, and NaOCl treatments were identified as adenylate kinase, phosphoglycerate kinase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and enolase, all of which are responsible for energy metabolism. Protein synthesis-associated proteins were downregulated and identified as elongation factor Tu and GAPDH. The inhibitory effects of SlAEW and AEW solutions against V. parahaemolyticus may be attributed to the changes in cell membrane permeability, protein synthesis activity, and adenosine triphosphate (ATP) biosynthesis pathways such as glycolysis and ATP replenishment.
Relation: 194 pp 529-537
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/52896
Appears in Collections:[食品科學系] 期刊論文

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