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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/52007

Title: Prokaryotic assemblages and metagenomes in pelagic zones of the South China Sea.
Authors: Ching-Hung Tseng
Pei-Wen Chiang
Hung-Chun Lai
Fuh-Kwo Shiah
Ting-Chang Hsu
Yi-Lung Chen
Liang-Saw Wen
Chun-Mao Tseng
Wung-Yang Shieh
Isaam Saeed
Saman Halgamuge
Sen-Lin Tang
Contributors: 國立臺灣海洋大學:海洋環境與生態研究所
Date: 2015
Issue Date: 2019-01-11T03:39:27Z
Publisher: BMC Genomics
Abstract: Abstract:
BACKGROUND:
Prokaryotic microbes, the most abundant organisms in the ocean, are remarkably diverse. Despite numerous studies of marine prokaryotes, the zonation of their communities in pelagic zones has been poorly delineated. By exploiting the persistent stratification of the South China Sea (SCS), we performed a 2-year, large spatial scale (10, 100, 1000, and 3000 m) survey, which included a pilot study in 2006 and comprehensive sampling in 2007, to investigate the biological zonation of bacteria and archaea using 16S rRNA tag and shotgun metagenome sequencing.

RESULTS:
Alphaproteobacteria dominated the bacterial community in the surface SCS, where the abundance of Betaproteobacteria was seemingly associated with climatic activity. Gammaproteobacteria thrived in the deep SCS, where a noticeable amount of Cyanobacteria were also detected. Marine Groups II and III Euryarchaeota were predominant in the archaeal communities in the surface and deep SCS, respectively. Bacterial diversity was higher than archaeal diversity at all sampling depths in the SCS, and peaked at mid-depths, agreeing with the diversity pattern found in global water columns. Metagenomic analysis not only showed differential %GC values and genome sizes between the surface and deep SCS, but also demonstrated depth-dependent metabolic potentials, such as cobalamin biosynthesis at 10 m, osmoregulation at 100 m, signal transduction at 1000 m, and plasmid and phage replication at 3000 m. When compared with other oceans, urease at 10 m and both exonuclease and permease at 3000 m were more abundant in the SCS. Finally, enriched genes associated with nutrient assimilation in the sea surface and transposase in the deep-sea metagenomes exemplified the functional zonation in global oceans.

CONCLUSIONS:
Prokaryotic communities in the SCS stratified with depth, with maximal bacterial diversity at mid-depth, in accordance with global water columns. The SCS had functional zonation among depths and endemically enriched metabolic potentials at the study site, in contrast to other oceans.
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/52007
Appears in Collections:[環境生物與漁業科學學系] 期刊論文

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