National Taiwan Ocean University Institutional Repository:Item 987654321/51939
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题名: Microbial community structure during oxygen-stimulated bioremediation in phenol-contaminated groundwater.
作者: Lin, Chi-Wen
Chi-Yung Lai
Li-Hsuan Chen
Wan-Fu Chiang
贡献者: 國立臺灣海洋大學:地球科學研究所
日期: 2007-02
上传时间: 2019-01-08T07:15:30Z
出版者: Journal of Hazardous Materials
摘要: Abstract: This research explored the changes in genetic diversity and spatial distribution of microbial communities in association with the changes in phenol concentration during a bioremediation process. Results using the traditional plate count method indicated an increase of average bacteria densities in groundwater from 10(4) to 10(7)CFUml(-1) initially to 10(7) to 10(9)CFUml(-1) after remediation. The diversity and stability of phenol-degrading bacterial communities were investigated by using single-strand-conformation polymorphism (SSCP) genetic profile analysis of 16S rDNA fragments amplified from groundwater samples. The molecular data showed a high degree of genetic similarity between communities from certain monitoring wells during the early phases of remediation, probably due to similar initial physical conditions among wells. Molecular signatures of several cultivated phenol-degrading bacterial strains could be seen in most groundwater profiles throughout the study period, suggesting that these strains were indigenous to the study site. It was also observed that the species diversity of these microbial communities increased as the phenol levels in the groundwater decreased during the 9-month study period, and recovered to the pre-treatment levels after the remediation program was completed.
關聯: 140(1-2) pp.221-229
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51939
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