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Title: Response surface optimization for ethanol production from Pennisetum Alopecoider by Klebsiella oxytoca THLC0409
Authors: Chi-Wen Lin
Dang-Thuan Tran
Chi-Yung Lai
Yet-Pole I
Chih-Hung Wu
Contributors: 國立臺灣海洋大學:地球科學研究所
Keywords: Cellulolytic
Direct microbial conversion
Ethanol production
Klebsiella oxytoca THLC0409
Pennisetum purpureum
Response surface methodology
Date: 2010-12
Issue Date: 2019-01-07T02:31:17Z
Publisher: Biomass and Bioenergy
Abstract: Abstract: The strain Klebsiella oxytoca THLC0409 was isolated from a lignocellulose-degrading microflora and used in the direct conversion of Napiergrass powder to ethanol. Central Composite Design (CCD), part of the Response Surface Methodology (RSM) was adopted to study the effects of the fermentation time, the initial pH of the liquid medium, the cultivation temperature, and the yeast extract (YE) concentration on ethanol production. A second-order model that relates the ethanol concentration to four basic factors was developed. Regression analysis revealed that the maximum ethanol concentration of 472 ppm could be reached at a fermentation time of about 11 days, an initial pH of 7.04, a cultivating temperature of 31 °C, and a YE concentration of 7.0 g l−1. The adjusted R-square of the regression model was 0.996, indicating a good fit at 99.6% confidence level. The highest ethanol yield and productivity were estimated to be 82 g kg−1 and 360 mg kg−1 h−1, respectively.
Relation: 34(12) pp.1922-1929
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51919
Appears in Collections:[應用地球科學研究所] 期刊論文

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