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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51823

Title: Elucidating in vitro cell-cell interaction using a microfluidic coculture system
Authors: Cheng-Wey Wei
Ji-Yen Cheng
Tai-Horng Young
Contributors: 國立臺灣海洋大學:機械與機電工程學系
Keywords: Microfluidic
Coculture
Cell-cell interaction
Date: 2006
Issue Date: 2018-12-25T07:54:48Z
Publisher: Biomedical Microdevices
Abstract: Abstract: This work presents a novel microfluidic coculture system that improves the accuracy of evaluating the interaction between cocultured cell types. A microfluidic coculture chip, fabricated by CO2 laser direct-writing on polymethyl methacrylate (PMMA), was designed to separate two cell types using a microchannel, while permitting transfer of cellular media. The system has two up-stream wells and five down-stream wells. As an example, released inflammatory cytokines (e.g., interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α)), activated in up-stream macrophages, flow through a microfluidic mixing system, generating linear concentration gradients in down-stream wells and inducing down-stream osteoblasts to release prostaglandin E2 (PGE2), a well-known bone resorption marker. Osteoblast viability was assessed by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay. This novel coculture system can be applied to evaluate cell-cell interaction while physically separating interacting cells.
Relation: 8(1) pp.65-71
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51823
Appears in Collections:[機械與機電工程學系] 期刊論文

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