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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51818

Title: A planar interdigitated ring electrode array via dielectrophoresis for uniform patterning of cells
Authors: Lo-Chang Hsiung
Chun-Hui Yang
Chi-Li Chiu
Chen-Lin Chen
Yueh Wang
Hsinyu Lee
Ji-Yen Cheng
Ming-Chih Ho
Andrew M.Wo
Contributors: 國立臺灣海洋大學:機械與機電工程學系
Keywords: Dielectrophoresis
Electrode array
Cell patterning
Cellular microarray
Date: 2008
Issue Date: 2018-12-25T07:20:31Z
Publisher: Biosensors and Bioelectronics
Abstract: Abstract: Uniform patterning of cells is highly desirable for most cellular studies involving cell–cell interactions but is often difficult in an in vitro environment. This paper presents the development of a collagen-coated planar interdigitated ring electrode (PIRE) array utilizing positive dielectrophoresis to pattern cells uniformly. Key features of the PIRE design include: (1) maximizing length along the edges where the localized maximum in the electric field exists; (2) making the inner gap slightly smaller than the outer gap in causing the electric field strength near the center of a PIRE being generally stronger than that near the outer edge of the same PIRE. Results of human hepatocellular carcinoma cells, HepG2, adhered on a 6 × 6 PIRE array show that cells patterned within minutes with good uniformity (48 ± 6 cells per PIRE). Cell viability test revealed healthy patterned cells after 24 h that were still confined to the collagen-coated PIREs. Furthermore, quantification of fluorescence intensity of living cells shows an acceptable reproducibility of cell viability among PIREs (mean normalized intensity per PIRE was 1 ± 0.138). The results suggest that the PIRE array would benefit applications that desire uniform cellular patterning, and improve both response and reproducibility of cell-based biosensors.
Relation: 24(4) pp.869-875
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51818
Appears in Collections:[機械與機電工程學系] 期刊論文

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