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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51817

Title: Label-free quantification of asymmetric cancer-cell filopodium activities in a multi-gradient chip.
Authors: Tsi-Hsuan Hsu
Meng-Hua Yen
Wei-Yu Liao
Ji-Yen Cheng
Chau-Hwang Lee
Contributors: 國立臺灣海洋大學:機械與機電工程學系
Date: 2009-01
Issue Date: 2018-12-25T06:50:25Z
Publisher: Lab-on-a-chip
Abstract: Abstract: We use novel super-resolution bright-field optical microscopy to observe the filopodium activities of human lung cancer cells in a multi-gradient cell culture chip. Temporal variations of the filopodium numbers are measured without fluorescent labelling. By carefully designing the fluidic field inside the culture chip, we establish stable concentration gradients of the injected reagents. The reagents are injected via a separated central inlet, and the concentration gradients are different at different positions in the chip. The same chip can be used for both control and treated experiments. Using epidermal growth factor as the treatment, we verify that the protrusions of filopodia indicate the direction of concentration gradients experienced by a living cancer cell; while the treatment of bovine serum albumin shows no specific effect on the growth of filopodia. The combination of label-free, high-resolution optical microscopy and a micro cell culture chip establishes a convenient and versatile platform for dynamical cancer-cell analyses.
Relation: 9(7) pp.884-890
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51817
Appears in Collections:[機械與機電工程學系] 期刊論文

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