English  |  正體中文  |  简体中文  |  Items with full text/Total items : 26987/38787
Visitors : 2217305      Online Users : 42
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search
LoginUploadHelpAboutAdminister

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51796

Title: Microfluidic ARray Synthesizer (MArS) for rapid preparation and hybridization of custom DNA microarray.
Authors: Ji-Yen Cheng
Huai-Yi Chen
Contributors: 國立臺灣海洋大學:機械與機電工程學系
Date: 2009-05
Issue Date: 2018-12-24T06:09:56Z
Publisher: Biotechnology and Bioengineering
Abstract: Abstract: The purpose of this work is to demonstrate a simple method that integrates custom DNA array synthesis and hybridization in a microfluidic channel. Commercial DLP projectors and a classical oligonucleotide synthesizer were integrated with a microfluidic chamber and constitute a Microfluidic ARray Synthesizer (MArS). Utilizing the benefit of microfluidics, the MArS produces two custom oligonucleotide arrays with reagent consumption for one array. The quality of the two arrays was examined by hybridization and shown to be almost identical. Post-synthesis deprotection of the array and hybridization performed in the same microfluidic chamber reduced overall DNA assay time by two orders. In addition, mismatch discrimination was enhanced using the microfluidic chamber. In summary, a simple method to build a custom array synthesizer is demonstrated and its performance is examined.
Relation: 104(2) pp.400-407
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51796
Appears in Collections:[機械與機電工程學系] 期刊論文

Files in This Item:

File Description SizeFormat
index.html0KbHTML5View/Open


All items in NTOUR are protected by copyright, with all rights reserved.

 


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback