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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51794

Title: Asymmetric cancer-cell filopodium growth induced by electric-fields in a microfluidic culture chip
Authors: C.-C. Wang
Y.-C. Ka
P.-Y. Chi
C.-W. Huang
J.-Y. Lin
C.-F. Chou
J.-Y. Cheng
C.-H. Lee
Contributors: 國立臺灣海洋大學:機械與機電工程學系
Date: 2010-11
Issue Date: 2018-12-24T03:46:30Z
Publisher: Lab-on-a-chip
Abstract: Abstract: We combine a micro-fluidic electric-field cell-culture (MEC) chip with structured-illumination nano-profilometry (SINAP) to quantitatively study the variations of cancer cell filopodia under external direct-current electric field (dcEF) stimulations. Because the lateral resolution of SINAP is better than 150 nm in bright-field image modality, filopodia with diameters smaller than 200 nm can be observed clearly without fluorescent labeling. In the MEC chip, a homogeneous EF is generated inside the culture area that simulates the endogenous EF environment. With this MEC chip-SINAP system, we directly observe and quantify the biased growth of filopodia of lung cancer cells toward the cathode. The epidermal growth factor receptors around the cell edges are also redistributed to the cathodal side. These results suggest that cancer-cell filopodia respond to the changes in EFs in the microenvironment.
Relation: 11(4) pp.695-699
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51794
Appears in Collections:[機械與機電工程學系] 期刊論文

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