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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51790

Title: In vitro electrical-stimulated wound-healing chip for studying electric field-assisted wound-healing process
Authors: Sun
Yung-Shin Peng
Shih-Wei Cheng
Contributors: 國立臺灣海洋大學:機械與機電工程學系
Date: 2012-08
Issue Date: 2018-12-24T03:32:06Z
Publisher: Biomicrofluidics
Abstract: Abstract: The wound-healing assay is an easy and economical way to quantify cell migration under diverse stimuli. Traditional assays such as scratch assays and barrier assays are widely and commonly used, but neither of them can represent the complicated condition when a wound occurs. It has been suggested that wound-healing is related to electric fields, which were found to regulate wound re-epithelialization. As a wound occurs, the disruption of epithelial barrier short-circuits the trans-epithelial potential and then a lateral endogenous electric field is created. This field has been proved invitro as an important cue for guiding the migration of fibroblasts, macrophages, and keratinocytes, a phenomenon termed electrotaxis or galvanotaxis. In this paper, we report a microfluidic electrical-stimulated wound-healing chip (ESWHC) integrating electric field with a modified barrier assay. This chip was used to study the migration of fibroblasts under different conditions such as serum, electric field, and wound-healing-promoting drugs. We successfully demonstrate the feasibility of ESWHC to effectively and quantitatively study cell migration during wound-healing process, and therefore this chip could be useful in drug discovery and drug safety tests.
Relation: 6(3)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51790
Appears in Collections:[機械與機電工程學系] 期刊論文

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