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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51789

Title: Electrotaxis of oral squamous cell carcinoma cells in a multiple-electric-field chip with uniform flow field.
Authors: Tsai
Hsieh-Fu Peng
Shih-Wei Wu
Chun-Ying Chang
Hui-Fang Cheng
Contributors: 國立臺灣海洋大學:機械與機電工程學系
Date: 2012-08
Issue Date: 2018-12-24T03:27:01Z
Publisher: Biomicrofluidics
Abstract: Abstract: We report a new design of microfluidic chip (Multiple electric Field with Uniform Flow chip, MFUF chip) to create multiple electric field strengths (EFSs) while providing a uniform flow field simultaneously. MFUF chip was fabricated from poly-methyl methacrylates (PMMA) substrates by using CO2 laser micromachining. A microfluidic network with interconnecting segments was utilized to de-couple the flow field and the electric field (EF). Using our special design, different EFSs were obtained in channel segments that had an identical cross-section and therefore a uniform flow field. Four electric fields with EFS ratio of 7.9:2.8:1:0 were obtained with flow velocity variation of only 7.8% CV (coefficient of variation). Possible biological effect of shear force can therefore be avoided. Cell behavior under three EFSs and the control condition, where there is no EF, was observed in a single experiment. We validated MFUF chip performance using lung adenocarcinoma cell lines and then used the chip to study the electrotaxis of HSC-3, an oral squamous cell carcinoma cell line. The MFUF chip has high throughput capability for studying the EF-induced cell behavior under various EFSs, including the control condition (EFS = 0).
Relation: 6(3)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51789
Appears in Collections:[機械與機電工程學系] 期刊論文

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