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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51782

Title: Electrokinetic acceleration of DNA hybridization in microsystems
Authors: Kin Fong Lei
Yun-Hsiang Wang
Huai-Yi Chen
Jia-Hong Sun
Ji-Yen Cheng
Contributors: 國立臺灣海洋大學:機械與機電工程學系
Keywords: DNA hybridization
Genetic diagnostics
Date: 2015-02
Issue Date: 2018-12-24T02:05:39Z
Publisher: Talanta
Abstract: Abstract: In this work, electrokinetic acceleration of DNA hybridization was investigated by different combinations of frequencies and amplitudes of actuating electric signals. Because the frequencies from low to high can induce different kinds of electrokinetic forces, i.e., electroosmotic to electrothermal forces, this work provides an in-depth investigation of electrokinetic enhanced hybridization. Concentric circular Cr/Au microelectrodes of 350 µm in diameter were fabricated on a glass substrate and probe DNA was immobilized on the electrode surface. Target DNA labeled with fluorescent dyes suspending in solution was then applied to the electrode. Different electrokinetic forces were induced by the application of different electric signals to the circular microelectrodes. Local microfluidic vortexes were generated to increase the collision efficiency between the target DNA suspending in solution and probe DNA immobilized on the electrode surface. DNA hybridization on the electrode surface could be accelerated by the electrokinetic forces. The level of hybridization was represented by the fluorescent signal intensity ratio. Results revealed that such 5-min dynamic hybridization increased 4.5 fold of signal intensity ratio as compared to a 1-h static hybridization. Moreover, dynamic hybridization was found to have better differentiation ability between specific and non-specific target DNA. This study provides a strategy to accelerate DNA hybridization in microsystems.
Relation: 138 pp.149-154
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51782
Appears in Collections:[機械與機電工程學系] 期刊論文

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