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http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51780
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| Title: | Uniform electric field generation in circular multi-well culture plates using polymeric inserts |
| Authors: | Hsieh-Fu Tsai
Ji-Yen Cheng
Hui-Fang Chang
Tadashi Yamamoto
Amy Q. Shen |
| Contributors: | 國立臺灣海洋大學:機械與機電工程學系 |
| Date: | 2016-05
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| Issue Date: | 2018-12-24T01:58:29Z
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| Publisher: | Scientific Report |
| Abstract: | Abstract: Applying uniform electric field (EF) in vitro in the physiological range has been achieved in rectangular shaped microchannels. However, in a circular-shaped device, it is difficult to create uniform EF from two electric potentials due to different electrical resistances originated from the length difference between the diameter of the circle and the length of any parallel chord of the bottom circular chamber where cells are cultured. To address this challenge, we develop a three-dimensional (3D) computer-aided designed (CAD) polymeric insert to create uniform EF in circular shaped multi-well culture plates. A uniform EF with a coefficient of variation (CV) of 1.2% in the 6-well plate can be generated with an effective stimulation area percentage of 69.5%. In particular, NIH/3T3 mouse embryonic fibroblast cells are used to validate the performance of the 3D designed Poly(methyl methacrylate) (PMMA) inserts in a circular-shaped 6-well plate. The CAD based inserts can be easily scaled up (i.e., 100 mm dishes) to further increase effective stimulation area percentages, and also be implemented in commercially available cultureware for a wide variety of EF-related research such as EF-cell interaction and tissue regeneration studies. |
| URI: | http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51780 |
| Appears in Collections: | [機械與機電工程學系] 期刊論文
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