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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51778

Title: Multiplex detection of urinary miRNA biomarkers by transmission surface plasmon resonance
Authors: Wing Kiu Yeung
Huai-Yi Chen
Juan-Jie Sun
Tung-Han Hsieh
Mansoureh Z. Mousavi
Hsi-Hsien Chen
Kuang-Li Lee
Heng Lin
Pei-Kuen Wei and Ji-Yen Cheng
Contributors: 國立臺灣海洋大學:機械與機電工程學系
Date: 2018-08
Issue Date: 2018-12-24T01:41:20Z
Publisher: Analyst
Abstract: Abstract: The clinical assessment of short-stranded nucleic acid biomarkers such as miRNAs could potentially provide useful information for monitoring disease progression, prompting definitive treatment decisions. In the past decade, advancements in biosensing technology have led to a shift towards rapid, real-time and label-free detection systems; as such, surface plasmon resonance (SPR) biosensor-based technology has become of high interest. Here, we developed an automated multiplex transmissive surface plasmon resonance (t-SPR) platform with the use of a capped gold nanoslit integrated microfluidic surface plasmon resonance (SPR) biosensor. The automated platform was custom designed to allow the analysis of spectral measurements using wavelength shift (dλ), intensity (dI) and novel area change (dA) for surface binding reactions. A simple and compact nanostructure based biosensor was fabricated with multiplex real-time detection capabilities. The sensitivity and specificity of the microfluidic device was demonstrated through the use of functionalised AuNPs for target molecule isolation and signal enhancement in combination with probes on the CG nanoslit surface. Our work allows for the multiplex detection of miRNA at femtomolar concentrations in complex media such as urine.
Relation: 19
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51778
Appears in Collections:[機械與機電工程學系] 期刊論文

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