English  |  正體中文  |  简体中文  |  Items with full text/Total items : 27287/39131
Visitors : 2445619      Online Users : 34
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search
LoginUploadHelpAboutAdminister

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51650

Title: Linearity improvement of Cy5 fluorescence concentration detection using series photodetector frequency circuit system with ITO-PolySi-Semiconductor-Metal photodetector
Authors: Yuh MingHsu
Chung ChengChang
Contributors: 國立臺灣海洋大學電機工程學系
Keywords: DNA probe
Fluorescence detection
Cy5
Laserphotodetector
Date: 2012-06
Issue Date: 2018-12-14T01:58:01Z
Publisher: SENSORS AND ACTUATORS A-PHYSICAL
Abstract: Abstract: In this study, the 48 MHz series photodetector frequency circuit system with ITO–PolySi–metal–semiconductor–metal photodetector was developed for Cy5 fluorescence detection of DNA probe ET996. The theory analysis of series photodetector frequency circuit system showed that the circuit parameters such as Cp and A (tan θ) can be applied to improve the frequency sensitivity. In this research, the lower capacitance of ITO–PolySi–metal–semiconductor–metal photodetector (Cp) was made and selected to improve the frequency sensitivity. According to derived theory of A adjustment, the capacitance match Cm and bias of ITO–PolySi–metal–semiconductor–metal photodetector were adjusted to improve the frequency sensitivity for detection of fluorescence dye concentration. On the basis of optimal conditions of capacitance match Cm and bias of photodetector, the detection limit of ET996-Cy5 fluorescence dye concentration 2 pmol/L can be measured by 48 MHz sensor system. Moreover, the detection linearity of 48 MHz sensor system was improved and the frequency shift ΔF was linearly related to the logarithm of fluorescence dye concentration in the range 2 pmol/L–20 μmol/L; meanwhile, through the feature of probe uniqueness, Edwardsiella BCRC 10670 DNA and fluorescence probe ET996-Cy5 can be successfully judged for hybridization reaction.
Relation: 124(21) pp.4963-4970
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51650
Appears in Collections:[電機工程學系] 期刊論文

Files in This Item:

File Description SizeFormat
index.html0KbHTML7View/Open


All items in NTOUR are protected by copyright, with all rights reserved.

 


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback