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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51276

Title: Purification and characterization of three amylases from viscera of hard clam
Authors: Ching‐Yu TSAO
Yen‐Hsin HSU
Li‐Min CHAO
Shann‐Tzong JIANG
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: amylase
characterization
enzyme
hard clam
Meretrix lusoria
purification
viscera
Date: 2004-02
Issue Date: 2018-11-21T07:25:11Z
Publisher: Fisheries Science
Abstract: Abstract: Three amylases were purified to electrophoretic homogeneity from viscera of hard clam Meretrix lusoria by ammonium sulfate fractionation, Sepharose 6B, DEAE‐Sephadex A‐50, Sephadex G‐200 and PBE 94 chromatographies. The purified amylases had molecular masses of 49.6, 58.7 and 100 kDa and were designated AI‐1, AI‐2 and AII, respectively. Both AI‐1 and AI‐2 could digest amylose into glucose and maltose, while AII could digest amylose and pullulan into glucose. The optimal pH and temperatures for AI‐1, AI‐2 and AII were 7.0, 7.5 and 7.5, and 40, 50 and 50°C, respectively. According to the substrate specificity, the purified AI‐1 and AI‐2 were considered to be multifunctional exo‐ and endo‐types of α‐amylase‐like enzymes, while AII was exo‐type γ‐amylase‐like enzyme. They were Ca2+‐independent enzymes.
Relation: 70(1) pp.174-182
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51276
Appears in Collections:[食品科學系] 期刊論文

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