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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51270

Title: Dietary copper requirement reevaluation for juvenile grouper, Epinephelus malabaricus, with an organic copper source.
Authors: Yu-Hung Lin
Chia-Chi Shih
Megan Kent
Shi-Yen Shiau
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: Grouper
Copper
Requirement
Organic form
Date: 2010-12
Issue Date: 2018-11-20T08:12:47Z
Publisher: Aquacultue
Abstract: Abstract: To reevaluate the dietary copper (Cu) requirements of grouper, Epinephelus malabaricus, with an organic Cu source, Cu peptide was added to a basal diet at 0, 1, 2, 3, 4, 8 and 12 mg Cu/kg, providing actual dietary values of 0.19, 1.81, 2.55, 3.43, 4.37, 8.99 and 12.81 mg Cu/kg for a total of 7 experimental diets. Each diet was fed to triplicate groups of fish (initial body weight: 21.60 ± 0.08 g) in a recirculating seawater rearing system for 8 wks. Weight gain (WG) was highest (P < 0.05) in fish fed the diet with 2.55 mg Cu/kg, followed by fish fed the diet with 3.43 mg Cu/kg, and lowest in fish fed diets with 0.19, 8.99 and 12.81 mg Cu/kg. Hepatic copper–zinc superoxide dismutase (Cu–Zn SOD) activity was highest in fish fed the diet with 2.55 mg Cu/kg, followed by fish fed diets with 3.43, 8.99, 1.81 and 12.81 mg Cu/kg (in order of decreasing activity), and lowest in fish fed the diet with 0.19 mg Cu/kg. Hepatic thiobarbituric acid-reactive substance (TBARS) value was lowest in fish fed diets with 2.55 and 3.43 mg Cu/kg, followed by fish fed diets with 0.19 and 4.37 mg Cu/kg, and highest in fish fed the diet with 12.81 mg Cu/kg. Analysis by broken-line regression of WG, hepatic Cu–Zn SOD activity and TBARS value, and by linear regression of whole-body Cu retention of the fish indicated that the adequate dietary Cu requirement for grouper determined by Cu-peptide is about 2–3 mg Cu/kg.
Relation: 310(1) pp.173-177
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51270
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