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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51231

Title: Modulation of nitrosative stress via glutathione-dependent formaldehyde dehydrogenase and S-nitrosoglutathione reductase
Authors: Ken CF
Huang CY
Wen L
Huang JK
Lin CT
Contributors: 國立臺灣海洋大學:生命科學暨生物科技學系
Date: 2014-08
Issue Date: 2018-11-19T08:22:34Z
Publisher: Int J Mol Sci.
Abstract: Abstract: Glutathione-dependent formaldehyde dehydrogenase (GFD) from Taiwanofungus camphorata plays important roles in formaldehyde detoxification and antioxidation. The enzyme is bifunctional. In addition to the GFD activity, it also functions as an effective S-nitrosoglutathione reductase (GSNOR) against nitrosative stress. We investigated the modulation of HEK (human embryonic kidney) 293T cells under nitrosative stress by transfecting a codon optimized GFD cDNA from Taiwanofungus camphorata (Tc-GFD-O) to these cells. The parental and transfected HEK 293T cells were then subjected to S-nitrosoglutathione treatment to induce nitrosative stress. The results showed that in Tc-GFD-O-transfected 293T cells, the expression and activity of GFD increased. Additionally, these cells under the nitrosative stress induced by S-nitrosoglutathione showed both higher viability and less apoptosis than the parental 293T cells. This finding suggests that the Tc-GFD-O in HEK 293T cells may provide a protective function under nitrosative stress.
Relation: 15(8) pp.14166-14179
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51231
Appears in Collections:[生命科學暨生物科技學系] 期刊論文

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