English  |  正體中文  |  简体中文  |  Items with full text/Total items : 26987/38787
Visitors : 2291759      Online Users : 34
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search
LoginUploadHelpAboutAdminister

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51100

Title: Evaluation of the Relationship Between the 18S rRNA/rDNA Ratio and Population Growth in the Marine Diatom Skeletonema tropicum via the Application of an Exogenous Nucleic Acid Standard
Authors: Yun‐Chi Lin
Lee‐Kuo Kang
Chi‐Yu Shih
Gwo‐Ching Gong
Jeng Chang
Contributors: 國立臺灣海洋大學:海洋環境與生態研究所
Keywords: Exogenous standards
growth rate
nitrogen
phosphorus
quantitative PCR
Date: 2018-04
Issue Date: 2018-11-12T06:12:10Z
Publisher: Journal of Eukaryotic Microbiology
Abstract: Abstract: Ribosomal RNA (rRNA) has been regarded as a proxy for metabolic activity and population growth in microbes, but the limitations and assumptions of this approach should be better defined, particularly in eukaryotic microalgae. In this study, the 18S rRNA/rDNA ratio of a marine diatom, Skeletonema tropicum, was examined in batch and semi‐continuous cultures subjected to low nitrogen and phosphorus treatments at a temperature of 20 °C. In the semi‐continuous cultures, the measured 18S rRNA/rDNA ratio ranged from 4.0 × 102 to 5.0 × 103, and the logarithmic form of this ratio increased linearly with the population growth rate under both low nitrogen and low phosphorus conditions. In batch cultures grown under low nitrogen or low phosphorus conditions, log (rRNA/rDNA) also increased linearly with growth rate when the latter ranged between −0.4 and 1.5 day−1. The 18S rRNA/rDNA ratios of Skeletonema sampled from in the southern East China Sea were substantially lower than measured from laboratory cultures. Among the field samples, ratios obtained at a coastal station were higher than those obtained farther offshore. These results imply higher growth rate at the coastal station, but the influences of other factors, such as cell size and temperature, cannot be ruled out.
Relation: 65(6) pp.792-803
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51100
Appears in Collections:[海洋環境與生態研究所] 期刊論文

Files in This Item:

File Description SizeFormat
index.html0KbHTML25View/Open


All items in NTOUR are protected by copyright, with all rights reserved.

 


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback