National Taiwan Ocean University Institutional Repository:Item 987654321/51097
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題名: Manipulation of fish germ cell: visualization, cryopreservation and transplantation.
作者: Tomoyuki Okutsu
Ayaka Yano
Kazue Nagasawa
Shinya Shikina
Terumasa Kobayashi,
Toshio Takeuchi
Goro Yoshizaki
貢獻者: 國立臺灣海洋大學:海洋環境與生態研究所
日期: 2006-12
上傳時間: 2018-11-12T05:52:38Z
出版者: Journal of Reproduction and Development.
摘要: Abstract: Germ-cell transplantation has many applications in biology and animal husbandry, including investigating the complex processes of germ-cell development and differentiation, producing transgenic animals by genetically modifying germline cells, and creating broodstock systems in which a target species can be produced from a surrogate parent. The germ-cell transplantation technique was initially established in chickens using primordial germ cells (PGCs), and was subsequently extended to mice using spermatogonial stem cells. Recently, we developed the first germ-cell transplantation system in lower vertebrates using fish PGCs and spermatogonia. During mammalian germ-cell transplantation, donor spermatogonial stem cells are introduced into the seminiferous tubules of the recipient testes. By contrast, in the fish germ-cell transplantation system, donor cells are microinjected into the peritoneal cavities of newly hatched embryos; this allows the donor germ cells to migrate towards, and subsequently colonize, the recipient genital ridges. The recipient embryos have immature immune systems, so the donor germ cells can survive and even differentiate into mature gametes in their allogeneic gonads, ultimately leading to the production of normal offspring. In addition, implanted spermatogonia can successfully differentiate into sperm and eggs, respectively, in male and female recipients. The results of transplantation studies in fish are improving our understanding of the development of germ-cell systems during vertebrate evolution.
關聯: 52(6) pp.685-693
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51097
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