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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51096

Title: Production of germ cell-deficient salmonids by dead end gene knockdown, and their use as recipients for germ cell transplantation.
Authors: Goro Yoshizaki
Kuniko Takashiba
Shotaro Shimamori
Kiyoko Fujinuma
Shinya Shikina
Tomoyuki Okutsu
Sachi Kume
Makoto Hayashi
Contributors: 國立臺灣海洋大學:海洋環境與生態研究所
Date: 2016-04
Issue Date: 2018-11-12T05:47:44Z
Publisher: Molecular Reproduction and Development
Abstract: Abstract: We previously established a spermatogonial transplantation model in fish using triploid recipients. Although triploid salmonids are sterile, they carry a limited number of immature triploid germ cells that potentially compete with the donor-derived germ cells for their niche. We therefore assessed the biological characteristics of germ cell-deficient gonads in rainbow trout for their suitability as recipients for germ cell transplantation in this study. Antisense morpholino oligonucleotides against the dead end gene were microinjected into the fertilized eggs of rainbow trout to eliminate endogenous germ cells, leaving only their supporting cells. Unlike similar approaches performed in zebrafish and medaka, these germ cell-deficient rainbow trout did not show a male-biased sex ratio. Approximately 30,000 spermatogonia were then transplanted into the body cavities of both germ cell-deficient and control recipients. The donor-derived germ cells showed significantly higher proliferation in the gonads of germ cell-deficient recipients than those in the gonads of the control recipients. Finally, the applicability of the germ cell-deficient recipients for xenogeneic transplantation was evaluated by transplanting rainbow trout spermatogonia into germ cell-deficient masu salmon recipients. The resulting recipient salmon matured normally and produced trout gametes, and early survival of the resulting trout offspring was as high as that of the control offspring. Thus, dead end-knockdown salmonids appear to be ideal recipients for the intraperitoneal transplantation of spermatogonia.
Relation: 83(4) pp.298-311
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/51096
Appears in Collections:[海洋環境與生態研究所] 期刊論文

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