Abstract: In this study, the complete mitogenome sequence of half-spined barbel Acrossocheilus hemispinus has been amplified and sequenced by employing long polymerase chain reaction method. The mitogenome, consisting of 16,590 base pairs (bp), had the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a noncoding control region (CR). CR of 940 bp length is located between tRNA(Pro) and tRNA(Phe). The overall base composition of A. hemispinus is 31.2% for A, 28.2% for C, 24.4% for T and 15.9 % for G, with a slight AT bias of 55.6%. The complete mitogenome may provide rather essential and important DNA molecular data for further phylogenetic analysis for not only congeneric species but also higher different taxa of Cyprinid fishes.