Abstract: In this study, the complete mitogenome sequence of balitorid fish, beautiful stone loach Traccatichthys pulcher (Nichols & Pope, 1927), collected from Hainan island, China, has been sequenced by the long polymerase chain reaction method. The mitogenome, consisting of 16,580 base pairs (bp), had typical vertebrate mitochondrial gene arrangement, including 13 protein coding genes, 22 transfer RNAs, 2 ribosomal RNA genes and a noncoding control region (CR). CR of 928 bp length is located between tRNAPro and tRNAPhe. The overall base composition of T. pulcher is 31.3% for A, 26.8% for C, 25.9% for T and 16.0% for G, with a slight AT bias of 57.3%. The complete mitochondrial genome may provide rather informative data for reconstructing and addressing new molecular perspectives for balitorid phylogenies.