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Title: Evaluation of antioxidative activity of Sargassum hemiphyllum ethanolic extract against hydrogen peroxide-induced injury in 3T3-L1 adipocytes
Authors: Yu-Lan Hung
Pai-An Hwang
Chwen-Herng Wu
Contributors: 國立臺灣海洋大學:生命科學暨生物科技學系
Date: 2012-06
Issue Date: 2018-10-15T08:15:50Z
Publisher: Journal of Taiwan Fisheries Research
Abstract: Abstract: In this study, the antioxidative and cytoprotective effect of Sargassum hemiphyllum ethanolic extract against hydrogen peroxide (H2O2)-stimulated 3T3-L1 adipocytes was investigated. The amount of total phenolic compounds in S. hemiphyllum ethanolic extract was 22.35 ± 1.41 mg/g dry seaweed. The antioxidative activity of S. hemiphyllum ethanolic extract was evaluated using four different methods, including reducing power, DPPH free radicals scavenging activity, Fe+2 chelating activity and SOD-like activity. The results showed that S. hemiphyllum ethanolic extract had the potential DPPH free radicals scavenging and SOD-like activities. The S. hemiphyllum ethanolic extract significantly reduced the intracellular reactive oxygen species (ROS) that produced by H2O2 stimulating, and it also raised cell survivability. At 1.5 and 2.0 mg/ml, S. hemiphyllum ethanolic extract increased the activities of antioxidant enzymes such as superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT), and decreased its mRNA expression of gp91phox and p67phox of NADPH oxidase subunits. Thus, it is suggested that S. hemiphyllum ethanolic extract increasingly protected 3T3-L1 adipocytes against oxidative damage by the cellular antioxidant activity and inhibited oxidase mRNA expression.
Relation: 20(1) pp.71-85
Appears in Collections:[Department of Bioscience and Biotechnology ] Periodical Articles

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