National Taiwan Ocean University Institutional Repository:Item 987654321/50532
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 28603/40634
造訪人次 : 4404747      線上人數 : 418
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 進階搜尋


題名: Peroxidase-mimicking PtNP-coated, 3D-printed multi-well plate for rapid determination of glucose and lactate in clinical samples
作者: Cheng-KuanSu
貢獻者: 國立臺灣海洋大學:生命科學暨生物科技學系
關鍵詞: Three-dimensional printing
Peroxidase mimic
Platinum nanoparticle
日期: 2018-09
上傳時間: 2018-10-15T01:56:32Z
出版者: Sensors and Actuators B: Chemical
摘要: Abstract: Three-dimensional printing (3DP) technologies provide great opportunities for prototyping devices with designed geometric functionality. As a further example of a multifunctional device manufactured using 3DP technologies, this paper describes a multi-well plate—fabricated using a fused deposition modeling-type 3D printer and then treated with a post-printing coating of peroxidase-mimicking platinum nanoparticles (PtNPs)—that (i) catalyzes the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (H2O2), (ii) allows determination of substances that can be oxidized by their specific oxidases to produce H2O2, and (iii) facilitates measurement of the absorbance of samples after direct loading into a plate reader. After method optimization, the analytical applicability of the PtNP-coated 3D-printed multi-well plate was illustrated in terms of reusability and stability, reaction kinetics, analytical performance, and the respective determination of glucose and lactate concentrations in urine, plasma, serum, and brain microdialysate samples. Such post-printing functionalization schemes should promote 3DP technologies for the future fabrication of multifunctional devices and an expansion of their practical applications.
關聯: 269 pp.46-53
顯示於類別:[生命科學暨生物科技學系] 期刊論文


檔案 描述 大小格式瀏覽次數



著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋