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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/50496

Title: Macroporous microbeads containing apatite-modified mesoporous bioactive glass nanofibres for bone tissue engineering applications
Authors: Hsu FY
Hsu HW
Chang YH
Yu JL
Rau LR
Tsai SW
Contributors: 國立臺灣海洋大學:生命科學暨生物科技學系
Keywords: Macropore
Microbead
Nanofibre
Mesoporous bioactive glass
Apatite
Date: 2018-08
Issue Date: 2018-10-12T06:21:11Z
Publisher: Materials Science and Engineering C
Abstract: Abstract: Mesoporous bioactive glass (MBG) has a greater surface area and pore volume than conventional BG. Hence, MBG is useful as a drug delivery carrier. Previously, MBG has been fabricated as dense or porous blocks. Compared to blocks, microbeads have a greater flexibility to fill different-shaped cavities with close packing. Moreover, fibrous materials have proven to increase cell attachment and differentiation because they mimic the three-dimensional structure of the natural extracellular matrix (ECM). Macroporous materials possess porous structures with interconnecting channels that allow the invasive growth of cells and capillaries. Hence, the aim of this study was to fabricate macroporous microbeads containing MBG nanofibres (MMBs). We used poly(methyl methacrylate) (PMMA) microspheres as the macroporous template in the process and removed the PMMA microspheres after the calcination treatment. Scanning electron microscopy imaging showed multiple pores on the surface of the MMBs, and a micro-computed tomography image showed the presence of pores throughout the entire microbead. The cellular attachment of MG63 osteoblast-like cells was considerably higher on the MMBs than on glass beads after culturing for 4 h. However, the cell viability greatly decreased after culturing for 1 day. We speculated that the release of a high concentration of calcium ions from the MMBs decreased the cell viability. To improve the cell viability, we modified the MMBs by immersing the MMBs in a simulated body fluid to fabricate a thin apatite layer on the surface of the MMBs. The apatite-modified MMBs (Ap-MMB) decreased the release of calcium ions and improved the cell viability. In an animal study, the bone defect in the control group did not recover. In contrast to the control group, the Ap-MMBs in the defect were nearly filled with new bone. The results show that the Ap-MMBs have great potential in osteogenesis for bone tissue engineering.
Relation: 89 pp.346-354
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/50496
Appears in Collections:[生命科學暨生物科技學系] 期刊論文

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