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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/50393

Title: Functional characterisation of a TLR accessory protein, UNC93B1, in Atlantic salmon (Salmo salar).
Authors: Lee PT
Zou J
Holland JW
Martin SA
Scott CJ
Kanellos T
Secombes CJ
Contributors: 國立臺灣海洋大學:水產養殖學系
Keywords: Accessory protein
Atlantic salmon
Gene expression
Innate immunity
TLR
UNC93B1
Date: 2015-01
Issue Date: 2018-10-08T01:44:40Z
Publisher: Dev Comp Immunol
Abstract: Abstract: Toll-like receptors (TLRs) are indispensable components of the innate immune system, which recognise conserved pathogen associated molecular patterns (PAMPs) and induce a series of defensive immune responses to protect the host. Biosynthesis, localisation and activation of TLRs are dependent on TLR accessory proteins. In this study, we identified the accessory protein, UNC93B1, from Atlantic salmon (Salmo salar) whole-genome shotgun (WGS) contigs aided by the conserved gene synteny of genes flanking UNC93B1 in fish, birds and mammals. Phylogenetic analysis showed that salmon UNC93B1 grouped with other vertebrate UNC93B1 molecules, and had highest amino acid identity and similarity to zebrafish UNC93B1. The salmon UNC93B1 gene organisation was also similar in structure to mammalian UNC93B1. Our gene expression studies revealed that salmon UNC93B1 was more highly expressed in spleen, liver and gill tissues but was expressed at a lower level in head kidney tissue in post-smolts relative to parr. Moreover, salmon UNC93B1 mRNA transcripts were up-regulated in vivo in spleen tissue from polyI:C treated salmon and in vitro in polyI:C or IFNγ stimulated Salmon Head Kidney-1 (SHK-1) cells. Initial studies into the functional role of salmon UNC93B1 in fish TLR signalling found that both wild type salmon UNC93B1 and a molecule with a site-directed mutation (H424R) co-immunoprecipitated with salmon TLR19, TLR20a and TLR20d. Overall, these data illustrate the potential importance of UNC93B1 as an accessory protein in fish TLR signalling.
Relation: 50(1) pp.38-48
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/50393
Appears in Collections:[水產養殖學系] 期刊論文

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