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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/50390

Title: Analysis of interferon gamma protein expression in zebrafish (Danio rerio).
Authors: Yoon S
Alnabulsi A
Wang TY
Lee PT
Chen TY
Bird S
Zou J
Secombes CJ
Contributors: 國立臺灣海洋大學:水產養殖學系
Keywords: Antigen re-stimulation
IFN-γ
Monoclonal antibody
PHA
Protein expression
Zebrafish
Date: 2016-10
Issue Date: 2018-10-08T01:22:05Z
Publisher: Fish Shellfish Immunol
Abstract: Abstract: IFN-γ is a major effector cytokine, produced to induce type I immune responses. It has been cloned in several fish species including zebrafish, however to date few studies have looked at IFN-γ protein expression and bioactivity in fish. Hence, the current study focused on developing a monoclonal antibody (moAb) against zfIFN-γ. We show that the zfIFN-γ moAb specifically recognises E. coli produced recombinant IFN-γ protein and zfIFN-γ produced in transfected HEK293 cells, by Western blot analysis. Next we analysed the production of the native protein following expression induced by PHA stimulation of leukocytes in vitro or antigen re-stimulation in vivo. We show the IFN-γ protein is produced as a dimer, and that a good correlation exists between transcript expression levels and protein levels.
Relation: 57 pp.79-86
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/50390
Appears in Collections:[水產養殖學系] 期刊論文

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