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|Title: ||Genetic diversity of a novel oil crop, Camellia brevistyla, revealed by ISSR DNA markers|
|Authors: ||Mong-Huai Su|
Khiem M. Nguyen
|Keywords: ||geographic regions|
|Issue Date: ||2018-09-28T08:16:50Z
|Publisher: ||Korean Journal of Horticultural Science & Technology|
|Abstract: ||Abstract: Camellia brevistyla plants are used as food oil in Taiwan. To provide a method for identifying
germplasm and assessing the genetic diversity of C. brevistyla, 102 individuals were collected
from 29 populations in 11 counties throughout the main island of Taiwan. Inter-simple sequence
repeat (ISSR) DNA markers were analyzed and a total of 176 bands were amplified. Of these,
175 bands were found to be polymorphic in these populations. Genetic similarities among
populations ranged between 34.1-95.1%. Cluster analysis revealed seven groups of populations
plus one outlier (C. kissii). Five individuals from two populations in the southern region were
grouped into cluster V, and six populations from both regions were grouped into cluster VII.
The remaining populations from the northern region were separated into different clusters from
V and VII. Results of an analysis of molecular variance indicated that the variation within
populations (75%) was predominantly greater than variations among counties (18%) and among
regions (7%). Overall, the gene flow (Nm = 0.509) estimated from genetic differentiation (Gst
= 0.534) suggested that gene flow among regions was limited. Higher genetic diversity (H =
0.317), Shannon’s index (I = 0.477), and gene flow (Nm = 0.538) of populations were found
in the northern region compared to the southern region. The DNA sequences of C. brevistyla
amplified by two oil-biosynthesis genes showed 99-100% homology with those of C. oleifera.
Camellia brevistyla populations are highly differentiated and can serve as a basis for the food
oil industry as a germplasm resource.
|Relation: ||35(5) pp.588-598|
|Appears in Collections:||[水產養殖學系] 期刊論文|
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