National Taiwan Ocean University Institutional Repository:Item 987654321/50167
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题名: Immunochemical and molecular characterization of a novel cell line derived from the brain of Trachinotus blochii (Teleostei, Perciformes): A fish cell line with oligodendrocyte progenitor cell and tanycyte characteristics
作者: Wen CM
Wang CS
Chin TC
Nan FH
Cheng ST
贡献者: 國立臺灣海洋大學:水產養殖學系
日期: 2010-06
上传时间: 2018-09-17T08:52:47Z
出版者: Send to Comp Biochem Physiol A Mol Integr Physiol.
摘要: Abstract: Ependymal radial glial cells, also called tanycytes, are the predominant glial fibrillary acidic protein (GFAP)- and vimentin (VIM)-expressing cells in fish ependyma. Radial glial cells have been proposed to be neural stem cells but their molecular expression is not well understood. Previous studies revealed that fish neural progenitor and neural stem cells have A2B5, a marker for oligodendrocyte progenitor cells (OPCs). In this study, an A2B5(+) cell line, SPB, was isolated from the brain of the teleost Trachinotus blochii and characterized. SPB cells usually grew as polygonal epithelial cells, but at high density, long processes were commonly observed. Using immunocytochemistry, SPB cells were shown to exhibit oligodendrocyte markers such as galactocerebroside and Olig2, and radial glial cell markers such as brain lipid-binding protein, GFAP, Sox2, and VIM. SPB cells were also observed to have DARPP-32, a marker for tanycytes in mammals, and primary cilia. RT-PCR additionally revealed expression of bone morphogenetic protein 4, connexin35, Noggin2, and proteolipid protein in SPB cells. Results of this study suggest that SPB cells are OPCs that can display tanycyte characteristics. Fish tanycytes can be neural stem cells suggesting that SPB cells are neural stem cells. SPB is the first fish cell line showing primary cilia and markers for both OPCs and tanycytes.
關聯: 156(2) pp.224-231
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/50167
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