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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/50153

Title: Characterization and virus susceptibility of a continuous cell line derived from the brain of Aequidens rivulatus (Günther).
Authors: Yeh SW
Nan FN
Cheng YH
Wen CM
Contributors: 國立臺灣海洋大學:水產養殖學系
Keywords: IPNV
cell line
cichlid
megalocytivirus
piscine reovirus
Date: 2018-04
Issue Date: 2018-09-17T07:40:18Z
Publisher: J Fish Dis
Abstract: Abstract: Cell cultures derived from the brain tissues of Aequidens rivulatus (Günther) have been characterized previously. In this study, a continuous cell line ARB8 was further established, and its growth characteristics, transcription and susceptibility to fish viruses-including chum salmon reovirus (CSV), marbled eel infectious pancreative necrosis virus (MEIPNV), grouper nervous necrosis virus (GNNV), giant seaperch iridovirus (GSIV), red seabream iridovirus (RSIV), koi herpesvirus (KHV), herpesvirus anguilla (HVA) and marbled eel polyoma-like virus (MEPyV)-were examined. ARB8 cells that showed epithelioid morphology and were passaged >80 times grew well at temperatures ranging from 25°C to 30°C in L-15 medium containing 5%-15% foetal bovine serum. The cells constitutively transcribed connexion 43, glutamine synthetase, nestin and nkx6-2, which are markers for neural progenitor cells. The cells were highly susceptible to CSV, MEIPNV, GSIV and RSIV and showed the typical cytopathic effect (CPE). However, the cells were resistant to GNNV, KHV, HVA and MEPyV because no significant CPE was noted after infection. Optimal temperatures for virus production ranged from 25°C to 30°C. The results revealed that the neural progenitor cell line ARB8 can potentially serve as a useful tool for investigating fish viruses and isolating new viruses in ornamental cichlid fishes.
Relation: 41(4) pp.635-641
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/50153
Appears in Collections:[水產養殖學系] 期刊論文

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