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|Title: ||Ultrastructure of Cell Wall Regeneration from Isolated Protoplasts of Grateloupia sparsa (Halymeniaceae, Rhodophyta)|
|Authors: ||Yean-Chang Chen|
Chiang, Y. M.
|Issue Date: ||2018-09-17T05:57:17Z
|Publisher: ||Botanica Marina|
|Abstract: ||Abstract: Ultrastructural observations were conducted on viable protoplast of Grateloupia sparsa isolated from the thallus by enzymatic degradation of the cell wall. Various stages (from 0 h to 168 h in culture) of protoplasts were examined under a SEM and a TEM. From the first six hours after isolation, the protoplasts showed a deposition of an amorphous matrix on the surface of the protoplasts. This substance is apparently produced in the endoplasmic reticulum, and is directly and continuously released to the surface. The protoplasts were fully covered by the amorphous matrix after one day in culture, as thereafter no obvious alteration was found on the surface of protoplasts before cell division was observed under SEM. Under TEM, the cell membrane of protoplasts was seen as smooth and intact without particular alteration before 6 h in culture; thereafter vesicles were excreted on the membrane. These vesicles formed an increasingly thick amorphous matrix. After about four days in culture, the cell membrane became irregular. At the same time, randomly arranged microfibrils were regenerated from this irregular membrane, these extended and later formed a thin layer of cell wall. The cell walls were continuously deposted, and at about day 7 in culture, the microfibrils became increasingly thick and ordered into arrays, and became involved with large amounts of terminal complex-like granules, before gradually forming a new complete cell wall.|
|Relation: ||38(5) pp.393-400|
|Appears in Collections:||[水產養殖學系] 期刊論文|
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