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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/50122

Title: Female-specific DNA sequences in ostriches
Authors: Wu CP
Horng YM
Yang KT
Huang CW
Huang MC
Contributors: 國立臺灣海洋大學:水產養殖學系
Date: 2006-10
Issue Date: 2018-09-17T02:11:21Z
Publisher: Molecular and Cellular Probes
Abstract: Abstract: Ostrich absence of heteromorphic sex chromosomes, unique sequences or markers located in the ostrich W-chromosome. Random amplified polymorphic DNA (RAPD) fingerprinting was carried out to investigate the sex-specific DNA sequence for sexing in ostrich. One hundred and forty random primers were used for random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). One of these primers, OPAJ-13, produced a sex-specific band only found in tested females, which was isolated and constructed into plasmids for nucleotide sequencing. A 760bp novel female-specific sequence was obtained. Two primers (OstSexOPAJ13-F and -R) were designed according to the cloned female sequence to amplify the female-specific fragment from genomic DNA of ostriches for sexing by PCR. The sex-specific band was represented in females but none were found in the males. This result showed that the sex of ostrich could be easily and effectively identified using the female-specific primers for PCR technique.
Relation: 20(5) pp.307-310
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/50122
Appears in Collections:[水產養殖學系] 期刊論文

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