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题名: DNA浮水印技術之研究與系統開發
A Study on DNA Watermark Technology and System Development
作者: Lin, Kun-You
林琨祐
贡献者: NTOU:Department of Computer Science and Engineering
國立臺灣海洋大學:資訊工程學系
关键词: DNA浮水印;加密;錯誤糾正碼;內含子;外顯子
DNA watermark;Encryption;ECC;Intron;Exon
日期: 2016
上传时间: 2018-08-22T06:57:02Z
摘要: 生物為求生存及繁衍後代,經由基因序列的複製並傳遞至子世代以達成生生不息的目的,該生物體的基因序列資訊可做為長期儲存個體基因性狀的媒介。若能將浮水印序列以人工方式嵌入於個體基因序列,使該序列檔案隨時隨地都能隱含使用者所加入具有可識別訊息的功能,或者讓活體生物的基因體序列攜帶重要的隱藏資訊,是目前後基因體世代在面對基因版權或基因序列個資防範議題的重要研究項目之一。 本研究提出一種針對基因序列加入浮水印的嵌入及解碼技術,分別使用基因序列的內含子與外顯子區域序列進行浮水印序列的嵌入及解碼,並根據生物不同特徵而選擇不同類型的嵌入模組。在內含子區域以直接鑲嵌序列方式將浮水印資訊嵌入於目標序列,該類技術可以插入相對大量的隱藏訊息;使用外顯子序列區域處理浮水印資訊的鑲嵌技術,主要是藉由胺基酸同義置換的特性嵌入浮水印資訊於目標中,並使其保有生物基因功能的相容性。嵌入的訊息同時使用進階加密標準進行鑲嵌浮水印序列的加密以加強隱藏訊息保密的安全性,最後透過錯誤校正碼與冗餘資訊驗證技術提高對抗基因隨機突變的雜訊及提升系統解碼的強健性。本基因浮水印加密系統集合編碼、解碼與錯誤碼檢測校正技術以強化系統的安全性及強健性,是一套可以落實DNA浮水印相關應用的完整系統。
To maintain the ability of an organism to survive and reproduce, gene transmission and inheritance to off-springs are primary principles of life sustainability. Thus, genome sequences of an organism can be considered as long-term storage media for individual genetic traits. If an artificial watermark sequence could be embedded within a gene sequence and enable the gene sequence possessing identifiable function at anytime from anywhere, or even make the gene sequences carry concealed information within a living organism, these applications have become the most important research issue for gene copyright or personal information protection in the post genome era. This work proposed a novel method to embed and decode DNA watermark sequence by inserting encrypted messages into genetic exon or intron regions annotated in a query DNA sequence. Based on the property of regions, two different embedding models could be selected. For intron region mechanisms, the watermark messages were embedded by inserting maximized carried messages. For exon region mechanisms, the watermark messages were embedded based on synonymous substitution to keep biocompatibility of organisms in nature. All embedded messages were encrypted by AES for enhancing security and added with ECC and redundant data for enhancing robustness to against the random substitution mutations. The proposed DNA watermark system is designed comprehensively for a fully functional system regarding security, robustness, and biocompatibility.
URI: http://ethesys.lib.ntou.edu.tw/cgi-bin/gs32/gsweb.cgi?o=dstdcdr&s=G0010157043.id
http://ntour.ntou.edu.tw:8080/ir/handle/987654321/49370
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