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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/48770

Title: 降低斑馬魚MicroRNA-27b以增加脂肪生成之功能性研究
Authors: Chen, Hsuan-chih
陳宣智
Contributors: 國立臺灣海洋大學:生命科學暨生物科技學系
Keywords: 斑馬魚;MicroRNA 27b;PPARγ;脂肪細胞;肥胖
zebrafish;MicroRNA 27b;PPARγ;adipocyte;obesity
Date: 2016
Issue Date: 2018-08-22T06:31:09Z
Abstract: 全球目前已有超過19億的成年人列為超重或是肥胖,也造成全球醫療上重要的問題,當脂肪累積過量時,對於健康造成許多負面影響,如糖尿病、心臟病,癌症風險等。而脂肪細胞的形成,主要受到脂肪儲存調節及環境因子所造成的影響,從以往的研究中指出miRNA可以加速或抑制脂肪細胞分化,並且調節其他基因的表現。對於脂肪分化的主要功能是由PPARγ所活化,然而mir27b是在PPARγ非編碼區上的作用位點。Mir27b的損害則會影響到PPARγ及C/EBP的標定物,本研究將透過miRNA-sponge技術,結合具有相同序列中多個miRNA合成,並且針對特異性結合miRNA進行相關的研究。研究中發現在Oil Red O stain時,高油脂餵食mir27b轉基因魚油滴分布較高於wild-type且有明顯差異,另外於RT-PCR半定量分析中,mir27b-sponge轉基因魚能使PPARγ及C/EBP等其脂肪代謝相關的target gene產生上調的趨勢。從H&E染色結果來看,發現在肝臟的部位,能清楚的觀察到mir27b相較於wild-type的脂肪細胞要來的大。結合以上結果得知mir27b確實在脂肪代謝及生成中扮演著重要的角色,因此希望未來能建立與脂肪代謝相關的疾病上能有更進一步的研究。
Obesity has emerged as a global health problem with more than 19 billion adults to be classified as overweight or obese, but also medically important global issue, when excess fat accumulation, and causing negative health effects. If excessive fat accumulation and for many negative health effects like diabetes, heart desease and cancer risk. From previous studies the mir27b may be to PPARγ non-coding regions on the action site, and through the mir27b damage will affect PPARγ and C/EBP calibration. Therefore, we chose MicroRNA-27b as the major research goals through RNA-sponge technology, combined with the same sequence number of miRNA in synthesis, research for specific binding miRNA. From the study found that in mir27b in the Zebrafish, obvious difference for fat build. In theory, mir27b sponge may induce activation of PPARγ, and generate more fat cell differentiation.In Oil o stain the study proved, fatty feeding mir27b sponge transgenic fish oil droplet of distribution is higher than wild type and gives it a significant difference. Beside the RT-PCR to half-quantitative mir27b sponge is able to do transgenic fish the PPARγ and C/EBP be released aim to fat metabolism in connection with trends, mir27b is adipose metabolisms and plays an important role build in him, so that fat metabolisms. Finally, we hope our model fish can provided a platform to study lipid accumulation and early stage adipocyte differentiation.
URI: http://ethesys.lib.ntou.edu.tw/cgi-bin/gs32/gsweb.cgi?o=dstdcdr&s=G0010336017.id
http://ntour.ntou.edu.tw:8080/ir/handle/987654321/48770
Appears in Collections:[生命科學暨生物科技學系] 博碩士論文

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