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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/48613

Title: 長額米蝦幼苗發育生物學研究暨鹽度與微藻對幼苗培育的影響
Authors: Chen, Chia-Yu
陳家瑜
Contributors: NTOU:Department of Aquaculture
國立臺灣海洋大學:水產養殖學系
Keywords: 長額米蝦(Caridina longirostris);幼苗發育;蝦苗培育;種蝦催熟;淡水馴化;微細藻
Caridina longirostris;Larva development;Larvae culture;Maturation induction;Freshwater acclimation;Mircroalgae
Date: 2017
Issue Date: 2018-08-22T06:06:41Z
Abstract: 本試驗主要目的為配合人工種蝦催熟,探討不同的培育鹽度、投餵不同的餌料生物,對長額米蝦初期蝦苗成長及發育的影響,以及後期蝦苗進行淡水馴化,藉以提供建立長額米蝦人工培育流程及商業化大量繁殖的參考。 長額米蝦幼苗階段包括眼幼蟲期(I~V)、糠蝦期(I~IV)及變態完成之後期幼蟲期,共10期。母蝦催熟試驗分為3組處理組(牡蠣、青蟲、烏賊)及控制組(商業飼料)進行8週飽食試驗,使母蝦性腺成熟的順序為青蟲及牡蠣處理組(6週)優於烏賊處理組(7週),而控制組(8週)最差。蝦苗鹽度試驗分為5組處理組(15、20、25、30、35‰)餵飼綜合微藻(2×105 cells/ml)及控制組(25‰,0 cells/m)進行試驗,蝦苗培育最適鹽度為20‰,活存率75.6±8.3%顯著高於25‰之58.9±5.1%與15‰之52.2±8.4%再高於35‰(47.8±6.9%)與30‰(44.4±5.1%);而蝦苗在鹽度25‰不投餵微藻下最多僅能存活6天。蝦苗培育餌料試驗分為6個微藻處理組(骨藻、周氏扁藻、魏氏海鏈藻、等邊金藻、牟氏角毛藻、骨藻+魏氏海鏈藻)進行試驗,餵飼骨藻+海鏈藻及骨藻之組別者蝦苗活存率最高 (43.7±2.3%、41.2±2.6%),其次為海鏈藻(22.8±6%)及扁藻(11.2±2%),等邊金藻與角毛藻活存率最低皆為0%;幼苗變態天數最短為骨藻+海鏈藻(19天)與骨藻(23天),其次為海鏈藻(25±1天),最久則是扁藻(32±2天);飼育扁藻、海鏈藻及等邊金藻的蝦苗有被藻類纏附而造成生長延遲甚至死亡的情形發生(卡藻)。淡水馴化試驗是將完成變態後的蝦苗分別留置於原20‰海水中1、3、6、10、20、30天後才開始進行淡水馴化,結果發現蝦苗在完成變態後1天內進行淡水馴化,平均活存率(75±10%)顯著低於變態後3天進行淡水馴化者(100%);因此最佳淡水馴化時機不可以在蝦苗完成變態的3天內進行。
The Caridina longirostris is a small sized species of aquarium shrimp together with its cousin the Amano shrimp (Caridina multidentata) as popular algae cleaners for aquarium. A series of experiments associated with maturation induction, larval culture and environmental condition have been conducted and concluded blow. Young adults fed polychaete exhibited the fastest gonado development within 6 weeks but showed no significance with those fed oyster. The overall result of dietary treatment on maturation induction effects compared was polychaete > oyster > squid > control. The survival rate (75.6±8.3%) of the metamorphosed post larvae at a salinity level of 20 ‰ was the highest among overall salinity conditions. Microalgae feeding trials showed that mixed algae (Skeletonema costatum + Thalassiosira weissflogii) yielded a better post larval harvest rate (43.7±2.3%) than any other trials by single algae species fed. Of the single algae comparisons, the post larval harvest rate of those fed Skeletonema costatum (41.2±2.6%) was the best, Thalassiosira weissflogii (22.8±6%) the second best, Tetraselmis chui being the moderate one (11.2±2%), while Isochrysis galabna (0%) and Chaetoceros muelleri (0%) were the lowest two. There requires at least a timing duration of 3 days post-staying in saline water before those metamorphosed juveniles can get through saline-freshwater acclimation transition process at the onset of the experiment to guarantee a full survivals of 100%.
URI: http://ethesys.lib.ntou.edu.tw/cgi-bin/gs32/gsweb.cgi?o=dstdcdr&s=G0010433022.id
http://ntour.ntou.edu.tw:8080/ir/handle/987654321/48613
Appears in Collections:[水產養殖學系] 博碩士論文

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