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Immediately Sex Reversal in the 17α-methyltestosterone-Induced Female Orange-Spotted Grouper, Epinephelus coioides
|Authors: ||Andri Iskandar|
|Contributors: ||NTOU:Department of Aquaculture|
|Issue Date: ||2018-08-22T06:06:13Z
The present study was expected to scrutinize the instrument of sex-change in fish in the early change process, whether the testis that is turned around from immature ovary using 17α-methyltestoterone (MT) would recover after the termination of MT treatment. We also connected MT-induced sex-change and 5-aza-2’-deoxycytidine (5-Aza) as DNA methylation inhibitor to comprehend the alternation of gonadal soma cells. In the first experiment, we used the orange-spotted groupers at the developmental ages and fed a diet containing MT at 50 mg/kg for 3 months and then a normal diet for 1 month. Intraperitoneally injection of 5-Aza was examined in the first week and second week during the MT-oral administration. Most of the fishes in the control group had immature ovaries, but all the females fed with MT, had an immature spermatogenesis. However, one month after the withdrawal of MT treatment, the sex of fish was returned to femaleness even the fish have undergone MT-induced masculinization. This outcome demonstrates precocious sex-change from underyearling orange-spotted grouper by means of oral MT treatment is impermanent. All the female of 5-aza treatments showed no spermatogenic cells. In this study, lower growth rates were demonstrated by the MT treated groups. The impact of this metabolic change was clear after the end of the hormone oral administration since the decreased growth of the groups treated for 3 months. The second experiment demonstrated the dmc1, nanos2 and sycp3 gene expression related to gonadal development to consider the cell-sexual re-programming of the cells in the AI/MT-terminated male-to-female sex-changed fish. Juvenile female fish were fed a diet containing AI/MT for 3 months and then AI/MT fed was terminated. To analyze the proliferating activities and cell tracing in the fish, we used BrdU injection at a different time. To obtain the gene expression of the fish gonad at different developmental days, we used q-PCR analysis. Our result revealed that during AI-induced sex-change, dmc1 genes showed significantly lower expression compared to the control fish in 0 d, 2 weeks and 8 weeks. The expression levels of dmc1 genes were increased in the MT-treated fish, and the differences were significant after 8 weeks of termination compared to the control fish. The higher expression of nanos2 genes showed at 4 weeks after termination in the MT treated fish
|Appears in Collections:||[水產養殖學系] 博碩士論文|
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