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The study of hypothalamus genes expression pattern during gonadal differentiation and sex change in black porgy, Acanthopagrus schlegelii
|Authors: ||Fan, Ching-Hsuan|
|Contributors: ||NTOU:Department of Aquaculture|
|Issue Date: ||2018-08-22T06:05:07Z
Black porgy is a protandrous hermaphroditic fish with bisexual gonads. During gonadal sex differentiation, the aromatase (cyp19a1b) had highly expression and the concentration of estradiol (E2) in the hypothalamus. However, the relationship between hypothalamus and gonad is still unknown. In this study, we use transcriptome to analyze the different stage of hypothalamus, including maleness (with bisexual gonads), femaleness (testis excised) and no sexuality (fish with no gonad). We obtain many genes (85 genes) have dimorphic expression in different groups. Therefore, we use these dimorphic expression genes to understand the brain sexuality during gonadal sex differentiation. According to the gonadal differentiation stage, the expression of target genes have three different patterns, including (i) lower expression after differentiation, (ii) highly expression after differentiation, and (iii) highly expression during differentiation time. Following these result, we are going to understanding the role of candidate genes of brain sexuality formation. For these reasons, we use drug to performed a E2-induced female (ovarian development) and AI- induced male to understand the possibility of brain sexuality. Because cyp19a1b expression showed a peak during gonad differentiation and sensitive to estrogen level. Thus, we use cyp19a1b and Amh try to find that genes are involved in gonad different without estrogen effects. We select 14 genes by the result and gene function. We also analyze the genes expression at both sexual phase, including male phase(1+-yr-old) and female phase(2+-yr-old). According to our results, we find 5 genes have significant highly expression in different gonad differentiation stage(male phase has 3 genes, passive female phase has 1 gene and potential male phase has 1 gene).
|Appears in Collections:||[水產養殖學系] 博碩士論文|
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