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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/47441

Title: Characterization and applications of single fluorescent nanodiamonds as cellular biomarkers
Authors: Wunshain Fann
Chi-Cheng Fu
Hsu-Yang Lee
Kowa Chen
Tsong-Shin Lim
Hsiao-Yun Wu
Po-Keng Lin
Pei-Kuen Wei
Pei-Hsi Tsao
Huan-Cheng Chang
Contributors: 國立臺灣海洋大學:光電科學研究所
Date: 2007-01
Issue Date: 2018-07-23T03:00:25Z
Publisher: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Abstract: Abstract: Type Ib diamonds emit bright fluorescence at 550–800 nm from nitrogen-vacancy point defects, (N-V)0 and (N-V)−, produced by high-energy ion beam irradiation and subsequent thermal annealing. The emission, together with noncytotoxicity and easiness of surface functionalization, makes nano-sized diamonds a promising fluorescent probe for single-particle tracking in heterogeneous environments. We present the result of our characterization and application of single fluorescent nanodiamonds as cellular biomarkers. We found that, under the same excitation conditions, the fluorescence of a single 35-nm diamond is significantly brighter than that of a single dye molecule such as Alexa Fluor 546. The latter photobleached in the range of 10 s at a laser power density of 104 W/cm2, whereas the nanodiamond particle showed no sign of photobleaching even after 5 min of continuous excitation. Furthermore, no fluorescence blinking was detected within a time resolution of 1 ms. The photophysical properties of the particles do not deteriorate even after surface functionalization with carboxyl groups, which form covalent bonding with polyL-lysines that interact with DNA molecules through electrostatic forces. The feasibility of using surface-functionalized fluorescent nanodiamonds as single-particle biomarkers is demonstrated with both fixed and live HeLa cells.
Relation: 104(3) pp.727-732
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/47441
Appears in Collections:[光電科學研究所] 期刊論文

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