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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/47428

Title: Study of Gold Nanoparticles and Live Cells Interactions by Using Planar Evanescent Wave Excitation.
Authors: Wei PK
Lee CW
Lin EH
Cheng JY
Contributors: 國立臺灣海洋大學:光電科學研究所
Date: 2009-03
Issue Date: 2018-07-18T08:50:46Z
Publisher: JOURNAL OF BIOMEDICAL OPTICS
Abstract: Abstract: We present a planar evanescent wave (PEW) technique combined with phase contrast optical microscopy to study the interactions between cells and gold nanoparticles (AuNPs). The PEW method employs a dual-fiber-line guide to couple light into a thin glass slide. It produces a uniform and long evanescent wave near the glass surface, as verified by the optical near-field measurement. High-contrast AuNP images are obtained by the PEW illumination. At the same time, cells are observed only by using the phase contrast microscopy. The nanoparticles and cell images indicated that unmodified AuNPs had no interactions with cells, possibly due to the negative surface charges on both cells and nanoparticles. The electrostatic concept was further verified by coating AuNPs with positively charged poly (L-lysine). DNA aptamers for surface mucin glycoprotein were coated on AuNPs to demonstrate the application for single nanoparticle tracking.
Relation: 14(2)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/47428
Appears in Collections:[光電科學研究所] 期刊論文

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