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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/47427

Title: Morphological Studies of Living Cells Using Gold Nanoparticles and Dark-Field Optical Section Microscopy
Authors: Wei PK
Lee CW
Chen MJ
Cheng JY
Contributors: 國立臺灣海洋大學:光電科學研究所
Date: 2009-05
Issue Date: 2018-07-18T08:45:24Z
Publisher: J. Biomedical Optics,
Abstract: Abstract: The morphologic changes of living cells under drug interactions were studied by using 80-nm gold nanoparticles and dark-field optical section microscopy. The gold nanoparticles were coated with poly (L-lysine), which attached to the membranes of various cells by way of electrostatic attractive force. A three-dimensional (3-D) morphological image was obtained by measuring the peak scattering intensities of gold nanoparticles at different focal planes. An algorithm for the reconstruction of 3-D cell morphology was presented. With the measured nanoparticle images and calculations, we show morphologic changes of lung cancer cells under the interaction of cytochalasin D drug at different times.
Relation: 14(3)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/47427
Appears in Collections:[光電科學研究所] 期刊論文

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