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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/47418

Title: Size-Dependent Endocytosis of Gold Nanoparticles Studied by Three-Dimensional Mapping of Plasmonic Scattering Images
Authors: Pei-Kuen Wei
Sheng-Hann Wang
Chia-Wei Lee
Arthur Chiou
Contributors: 國立臺灣海洋大學:光電科學研究所
Keywords: Dielectric Sphere
Gold Nanoparticles
Localise Surface Plasmon Resonance
Cellular Organelle
Chloroauric Acid
Date: 2010-12
Issue Date: 2018-07-18T07:12:12Z
Publisher: J Nanobiotechnology
Abstract: Abstract: Background:Understanding the endocytosis process of gold nanoparticles (AuNPs) is important for the drug delivery and photodynamic therapy applications. The endocytosis in living cells is usually studied by fluorescent microscopy. The fluorescent labeling suffers from photobleaching. Besides, quantitative estimation of the cellular uptake is not easy. In this paper, the size-dependent endocytosis of AuNPs was investigated by using plasmonic scattering images without any labeling.
Results:The scattering images of AuNPs and the vesicles were mapped by using an optical sectioning microscopy with dark-field illumination. AuNPs have large optical scatterings at 550-600 nm wavelengths due to localized surface plasmon resonances. Using an enhanced contrast between yellow and blue CCD images, AuNPs can be well distinguished from cellular organelles. The tracking of AuNPs coated with aptamers for surface mucin glycoprotein shows that AuNPs attached to extracellular matrix and moved towards center of the cell. Most 75-nm-AuNPs moved to the top of cells, while many 45-nm-AuNPs entered cells through endocytosis and accumulated in endocytic vesicles. The amounts of cellular uptake decreased with the increase of particle size.
Conclusions:We quantitatively studied the endocytosis of AuNPs with different sizes in various cancer cells. The plasmonic scattering images confirm the size-dependent endocytosis of AuNPs. The 45-nm-AuNP is better for drug delivery due to its higher uptake rate. On the other hand, large AuNPs are immobilized on the cell membrane. They can be used to reconstruct the cell morphology.
Relation: 8(1)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/47418
Appears in Collections:[光電科學研究所] 期刊論文

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