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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/47381

Title: Chromatogram Analysis on Revealing Aggregated Number and Location of Gold Nanoparticles Within Living Cells
Authors: Pei-Kuen Wei
Sheng-Hann Wang
Chia-Wei Lee
Ming-Yang Pan
Shu-Yi Hsieh
Fan-Gang Tseng
Contributors: 國立臺灣海洋大學:光電科學研究所
Keywords: Color transform
Gold nanoparticles
Living cells
Scattering image
Date: 2014-12
Issue Date: 2018-07-17T05:55:17Z
Publisher: Plasmonics
Abstract: Abstract: We present a method to directly observe three-dimensional (3D) distribution of aggregated gold nanoparticles (Au NPs) in live cells using dark-field sectional optical microscopy with chromatographic analysis. The scattering images of clustered Au NPs were transformed from RGB regular coordinate to hue, saturation, and value (HSV) cylindrical coordinate. The hue distinguished Au NPs from scattering images of cellular organelles. The saturation eliminated the interference from the defocused spots. The value, which indicated the scattering intensity, defined the 3D locations of Au NPs. The V/H ratio showed a linear relation with the Au NP number in the cluster. It was applied to estimate the numbers of aggregated nanoparticles in endosome vesicles. Using the chromatographic analysis, the calculated nanoparticle numbers were in good agreement with mass spectroscopic measurement. Compared to conventional methods, the chromatographic approach provides a simple observation of 3D distribution of Au NPs and their aggregation states in living cells.
Relation: 10(4) pp.873–880
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/47381
Appears in Collections:[光電科學研究所] 期刊論文

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