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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/47376

Title: Label-Free Detection of Rare Cell in Human Blood Using Gold Nano Slit Surface Plasmon Resonance
Authors: Ji-Yen Cheng
Mansoureh Z. Mousavi
Huai-Yi Chen
Hsien-San Hou
Chou-Yuan-Yuan Chang
Steve Roffler
Pei-Kuen Wei
Contributors: 國立臺灣海洋大學:光電科學研究所
Date: 2015-05
Issue Date: 2018-07-17T03:37:43Z
Publisher: BIOSENSORS
Abstract: Abstract: Label-free detection of rare cells in biological samples is an important and highly demanded task for clinical applications and various fields of research, such as detection of circulating tumor cells for cancer therapy and stem cells studies. Surface Plasmon Resonance (SPR) as a label-free method is a promising technology for detection of rare cells for diagnosis or research applications. Short detection depth of SPR (400 nm) provides a sensitive method with minimum interference of non-targets in the biological samples. In this work, we developed a novel microfluidic chip integrated with gold nanoslit SPR platform for highly efficient immunomagnetic capturing and detection of rare cells in human blood. Our method offers simple yet efficient detection of target cells with high purity. The approach for detection consists of two steps. Target cells are firs captured on functionalized magnetic nanoparticles (MNPs) with specific antibody I. The suspension containing the captured cells (MNPs-cells) is then introduced into a microfluidic chip integrated with a gold nanoslit film. MNPs-cells bind with the second specific antibody immobilized on the surface of the gold nanoslit and are therefore captured on the sensor active area. The cell binding on the gold nanoslit was monitored by the wavelength shift of the SPR spectrum generated by the gold nanoslits.
Relation: 5(1)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/47376
Appears in Collections:[光電科學研究所] 期刊論文

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