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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/47371

Title: Nanoplasmonic biochips for rapid label-free detection of imidacloprid pesticides with a smartphone
Authors: Pei-Kuen Wei
Kuang-Li Lee
Meng-Lin You
Chia-Hsin Tsai
En-Hung Lin
Shu-Yi Hsieh
Ming-Hsun Ho
Ju-Chun Hsu
Contributors: 國立臺灣海洋大學:光電科學研究所
Keywords: Surface plasmon resonance
Imidacloprid insecticide
Label-free detection
Optical biosensor
Spectral image
Date: 2016-01
Issue Date: 2018-07-17T02:55:37Z
Publisher: Biosensors and Bioelectronics
Abstract: Abstract: The widespread and intensive use of neonicotinoid insecticides induces negative cascading effects on ecosystems. It is desirable to develop a portable sensitive sensing platform for on-site screening of high-risk pesticides. We combined an indirect competitive immunoassay, highly sensitive surface plasmon resonance (SPR) biochip and a simple portable imaging setup for label-free detection of imidacloprid pesticides. The SPR biochip consists of several capped nanoslit arrays with different periods which form a spectral image on the chip. The qualitative and semiquantitative analyses of pesticides can be directly observed from the spot shift on the chip. The precise semiquantitative analyses can be further completed by using image processing in a smartphone. We demonstrate simultaneous detection of four different concentrations of imidacloprid pesticides. The visual detection limit is about 1ppb, which is well below the maximum residue concentration permitted by law (20ppb). Compared to the one-step strip assay, the proposed chip is capable of performing semiquantitative analyses and multiple detection. Compared to the enzyme-linked immunosorbent assay, our method is label-free and requires simple washing steps and short reaction time. In addition, the label-free chip has a comparable sensitivity but wider working range than those labeling techniques.
Relation: 75(15) pp.88-95
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/47371
Appears in Collections:[光電科學研究所] 期刊論文

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